Anti-obesity drugs – a group of brain cells controls energy expenditure

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Effective weight-loss strategies call for eating less food, burning more calories – or ideally, both.

But for the more than 90 million Americans who suffer from obesity, a disease that contributes to conditions ranging from cancer to heart disease, behavioral change is hard to accomplish or not effective enough – which is why scientists have long sought drugs that would help people shed pounds.

Yet effective, long-lasting treatments have thus far eluded them.

In a new report published in Cell, researchers in the laboratory of Jeffrey M. Friedman propose a new avenue in the search for anti-obesity drugs.

Collaborating with a Princeton University team, they have found that a group of brain cells previously shown to regulate hunger also controls energy expenditure.

And since our body weight depends both on the calories we consume and the energy we burn, these findings could lead to a new type of weight-loss medication that acts on both sides of the energy equation.

Energy in, energy out

Thus far, most obesity research has focused on the biological mechanisms that govern how much we eat.

Yet manipulating the neural circuits that control our calorie intake hasn’t led to broadly successful anti-obesity drugs.

So, Marc Schneeberger Pané, a postdoctoral Kavli NSI fellow in Friedman’s lab, launched a project to investigate the processes by which we burn energy instead.

Mammals like mice and humans expend energy in many ways, and producing heat is among most important. When the ambient temperature drops, we burn more fuel to maintain a steady body temperature; when it rises, we burn less.

We even possess a special form of fat tissue, known as brown fat, that is burned to produce heat directly.

Scientists knew that some populations of temperature-sensitive neurons in the hypothalamus region of the brain play a role in regulating heat production and, therefore, energy expenditure.

But they did not know exactly how those neurons exerted their influence, or if other cells outside the hypothalamus might play a similar role.

Double duty

Schneeberger Pané and his colleagues, including graduate student Luca Parolari, began by mapping the brain regions activated by a rise in ambient temperature.

They used an advanced 3-D-imaging technique called iDISCO, developed at Rockefeller, to scan the brains of mice exposed to hot temperatures, looking for signs of neuronal activity.

As expected, the team saw activity in the hypothalamus.

But they also saw activity among a particular group of neurons in a part of the brainstem known as the dorsal raphe nucleus – and, to the researchers’ surprise, these happened to be the very same neurons that, just two years earlier, the lab had found to be crucial for controlling hunger.

“Our new findings demonstrate that these neurons regulate energy balance by modulating both food intake and energy expenditure through partially overlapping circuit mechanisms,” says Alexander R. Nectow, who led the Princeton University team.

The possibility that these cells, which the lab had previously dubbed “hunger neurons,” might regulate both hunger and energy expenditure raised the prospect that they could serve as powerful levers for managing body weight.

“We were very excited,” recalls Schneeberger Pané, who adds that he and his colleagues view these multitalented neurons as “a new horizon in obesity research.”

The researchers used sophisticated biochemical techniques to alternately turn these temperature-sensitive brainstem neurons on and off.

They discovered that activating the neurons reduced the temperature of the animals’ brown fat, which is burned to generate heat, core body temperature as well.

Suppressing the neurons, meanwhile, amped up heat production – and, as the scientists had shown previously, it also made the animals less hungry.

Eat less, burn more

But generating heat by burning brown fat isn’t the only way to expend energy.

Physical activity burns calories too, as do all the basic tasks that keep the body alive: breathing, digesting food, and so on.

So the researchers put the mice in special cages tricked out with sensors to track their movements and gauge how much carbon dioxide they produce and how much food, water, and oxygen they consume.

The goal was to see if the temperature-sensitive hunger neurons dorsal raphe nucleus could control energy expenditure not only by regulating temperature, but by other means as well.

Again, the results were clear: just as activating the neurons caused heat production to plummet, so too did it cause movement, metabolic activity, and overall energy expenditure to nosedive.

Suppressing the neurons, on the other hand, caused all of them to rise.

The team has already begun searching for unique receptors in these dual-purpose neurons that govern both hunger and energy expenditure.

The idea is to identify targets that can be used to create novel anti-obesity drugs capable of delivering a one-two punch.

“When you inhibit these neurons, they suppress food intake and increase energy expenditure at the same time,” says Schneeberger Pané.

Figuring out how to quiet them in people could enable a doubly effective assault against a massive public health problem.


A paradigm shift for the prevention and treatment of obesity

The prevalence of obesity and overweight has plateaued in developed countries, albeit at high levels, but continues to rise in many parts of the world.1,2 

Current recommendations for preventing and treating obesity are mainly based on the notion that overeating results from hedonic eating as a result of unlimited access to palatable foods, particularly those high in sugar and fat, and that hedonic centers are able to “override” the body’s homeostatic mechanisms.3

It is undeniable that hedonic mechanisms can lead to episodes of overeating.

This article proposes, however, that the homeostatic mechanisms affecting appetite and satiety are more important in chronic overeating and the development of obesity.

Although the physiology underlying these homeostatic mechanisms is still not fully understood, sufficient evidence exists for adopting a new paradigm for individual and global weight control that is directed at the enteroendocrine and gut microbiota systems controlling energy homeostasis.46

Obesity as a chronic disorder of hunger and satiety

Two types of brain centers regulate food intake. Short- and long-term energy homeostasis is controlled by centers in the hypothalamus and brainstem that receive hormonal and vagal signals relating to the body’s metabolic status and the nutrient content of individual meals.

The hormones cholecystokinin, glucagon-like peptide (GLP-1), peptide tyrosine tyrosine (PYY) and nesfatin-1, derived from the gastrointestinal tract, have local and central actions and inhibit food intake by crossing the blood–brain barrier and targeting the arcuate nucleus of the hypothalamus.

The gastrointestinal-derived peptide ghrelin has peripheral and central actions that stimulate food intake.

Messages from the vagus nerve reach the solitary nucleus in the medulla oblongata via the solitary tract, and nerve fibers then project from this nucleus to other areas of the brain. Non-homeostatic hedonic aspects of eating are controlled by centers in the cortex, basal ganglia and limbic system that involve reward-motivated, cognitive and emotional factors in response to visual, olfactory and orosensory cues.7 

Both types of centers communicate with each other via neural pathways and both respond to endocrine signals.3,4,8,9 

A magnetic resonance imaging study on sucrose ingestion has suggested greater connectivity between homeostatic and hedonic centers in the obese than in the lean, indicating the possibility that hedonic mechanisms may be enhanced in the obese in the presence of an underlying homeostatic abnormality.10

Obese individuals eat larger meals than people of normal weight.1113 

Evidence that this is often due to a persistent hyperphagic state comes from a study in the US that provided questionnaires to the parents of obese children and adolescents.14 

The parents were requested that these be completed together with their offspring, and they included questions about the child’s hunger on a 5-point scale (“eats less than others” to “eating a lot, always abnormally hungry”) and speed of eating on a 4-point scale (“very slow” to “very fast”).

Of the 127 obese patients with a body mass index (BMI) greater than the 95th percentile, 62.2% reported “eating a lot and always abnormally hungry” or “eating a lot and often abnormally hungry” compared to 21.4% of normal weight children with a BMI <75th percentile.

Also, 55.7% of the obese patients admitted to eating “very fast” or “moderately fast” vs 23.3% of controls.

Both differences were highly significant (P<0.001). Hunger and speed of eating were also highly correlated (P<0.001), suggesting that eating fast was part of this hyperphagic state.

This study was repeated in a weight control clinic for children and adolescents in Jerusalem, Israel, and the results were very similar.

Of 100 Jewish and Arab obese pediatric patients, 66% had hunger recognized by the families as abnormal on a 7-point scale and 45% of these 100 patients had the highest rating, that is, “hunger for most of the time” (AH Slyper, personal communication, November 2018).

Hunger of this degree is quite unusual in normal weight children, being seen in only 3 of 71 normal weight controls, and is far more indicative of a persistent homeostatic abnormality than an intermittent hedonic one.

It could be argued that hunger is too subjective a phenomenon to be evaluated meaningfully by a questionnaire.

However, the parents’ answer to this question reflected their perceptions of how their child usually behaves in relation to food and can be regarded as reasonably objective.

The studies in the two countries were performed in community pediatric endocrine centers and the children seen were representative of obese children in these communities.


More information: Marc Schneeberger et al, Regulation of Energy Expenditure by Brainstem GABA Neurons, Cell (2019). DOI: 10.1016/j.cell.2019.05.048

Journal information: Cell
Provided by Rockefeller University

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