The genus Rhodiola (Hong Jing Tian; Crassulaceae) consists of more than 200 species, of which approximately 20, including Rhodiola rosea, Rhodiola alterna, Rhodiola brevipetiolata, Rhodiola crenulata, Rhodiola kirilowi, Rhodiola quadrifida, Rhodiola sachalinensis, and Rhodiola sacra, are used as traditional medicines in Asia . These plants grow mainly in the Himalayan belt, Tibet, China, and Mongolia, but are also cultivated in Europe and North America and are available on the market as dietary supplements , .
Rhodiola plants extracts are traditionally used in tonics and adaptogen, antidepressant, and anti-inflammatory drugs , . The most widely known is Rhodiola rosea, which is also called the “golden root” or “roseroot.” The pharmacological effects of Rhodiola rosea, including its role in increasing longevity, stimulating the central nervous system, and elevating work performance as well as its cardio-, neuro-, and hepatoprotective effects and immunotropic, antiviral, anti-inﬂammatory, and antibacterial activities, have been studied extensively , .
Rhodiola rosea has been used for a long time in Eastern Europe and Asia to enhance physical and mental performance. It is also used in Eastern Europe and Asia as a traditional medicine to stimulate the nervous system, alleviate depression and fatigue, enhance work performance, and prevent high-altitude sickness, mountain malhypoxia, and anoxia , and in Russia and Mongolia for treating long-term illness and weakness caused by infection .
In addition, Rhodiola rosea has been proven to have cardiovascular protection effects , . In recent years, the root extracts of Rhodiola rosea have been used as drinks, food additives, and in commercial pharmaceutical preparations offered worldwide .
Rhodiola plants contain polyphenols such as ﬂavonoids, proanthocyanidines, tyrosol, and cinnamyl alcohol, as well as glycosides, organic acids, essential oils, sugars, fats, alcohols, and proteins .
The polyphenol content of Rhodiola rosea is approximately 41.4 ± 3.41% . Rosavin (such as rosavin, rosarin, rhodionin, rhodiosin, and rosin), cinnamyl alcohol, salidroside (Fig. 1B), and tyrosol (Fig. 1A) are the major components of Rhodiola plants , , , , , , . Salidroside and its aglycone, tyrosol, are the major compounds of Rhodiola rosea, and the content of the two compounds are often used as a criterion in evaluating the quality of crude drugs of Rhodiola rosea.
This review article presents an overview of the bioactivities of Rhodiola plants and their major components.
The genus Rhodiola in the family Crassulaceae contains herbaceous perennial plants that often occur as fleshy creeping rhizomes. Rhodiola species are also commonly known as rosenroot, golden root and orpin rose.
Throughout history, they have been considered valuable medicinal plants in China, Europe, and North America.
There are 96 species of Rhodiola in the world and most are found in different regions of China.
China is considered the main growing area of Rhodiola with 73 species, two subspecies and seven varieties.
Within China, about 90% of Rhodiola can be found in the northwest, southwest and northeast regions such as Tibet, Qinghai, Yunnan, Sichuan and other alpine provinces. In the plant classification system, genera have several subcategories including sections, series and species. According to the “Chinese flora”, the genus Rhodiola has eight sections.
The medicinal herbs in this genus are primarily found in three sections: Sect. Chamaerhodiola (Fisch. et Mey.) A. Bor., Sect. Rhodiola, and Sect. Trifida (Frod.) S. H. Fu. The main medicinal Rhodiola species are listed in Table 1.
Rhodiola usually grows in limestone and granite soils at high altitudes (3500 to 5000 m), although a few species can also be found in alpine grasslands or shrublands at altitudes of about 2000 m.
Species, geographical distributions, and growing environments of medicinal Rhodiola.
|Section||Series||Latin Name||Geographical Origin||Growing Environment||Altitude (m)|
|Sect. Chamaerhodiola (Fisch. et Mey.) A. Bor.||Ser. Dumulosae (Frod.) S.H. Fu||R. dumulosa (Franch.) S.H. Fu||Sichuan, Shanxi, Gansu, Ningxia, Qinghai, Shanxi, Hebei, Inner Mongolia||Slopes, rocks||1600-3900|
|Ser. Quadrifidae (Frod.) S.H. Fu||R. quadrifida (Pall.) Fisch. et Mey.||Tibet, Sichuan, Xinjiang, Gansu, Qinghai||Alpine meadows, schist on mountain slopes, rock crevices on mountain slopes, marshes||3000-5700|
|R. scabrida (Franch.) S.H. Fu||Sichuan, Yunnan||Grassland on slopes||3200-4700|
|R. subopposita (Maxim.) Jacobsen||Gansu, Qinghai||Rock crevices on mountain slopes||1600-5000|
|R. atuntsuensis (Praeg.) S.H. Fu||Yunnan|
|Ser. Fastigiatae (Frod.) S.H. Fu||R. fastigiata (Hook. f. et Thoms.) S.H. Fu||Tibet, Sichuan, Gansu, Yunnan||Schist on mountain slopes, slopes, rock crevices||3300-5400|
|R. pamiroalaica A. Bor.||Xinjiang||–||2000-4200|
|R. himalensis (D. Don.) S.H. Fu||Sichuan||–||3700-4200|
|R. tangutica (Maxim.) S.H. Fu||Sichuan, Gansu, Ningxia, Qinghai||Rock crevices on mountain slopes, meadows, around water||2000-4700|
|Sect. Rhodiola||Ser. Roseae (Praeg.) S.H. Fu||R. rosea L.||Xinjiang, Qinghai||Alpine grasslands, under forest, beside ditches||1800-2035|
|R. sachalinensis A. Bor||Heilongjiang, Jilin||Under hills and trees, under rocks||1700-2300|
|R. crenulata (Hook. f. et Thomas) H. Ohba||Tibet, Sichuan, Qinghai||Alpine gravel beach, slopes, grasslands, rock crevices||3400-5600|
|R. kirilowii (Regel) Maxim.||Qinghai, Sichuan, Xinjiang, Shanxi, Gansu||Schist on mountain slopes, under rocks in the forest, meadows, beside ditches||3100-5600|
|R. linearifolia A. Bor||Xinjiang||–||2000-4200|
|Ser. Bupleuroides Frod.) S.H. Fu||R. bupleuroides (Wall. ex Hook. f. et Thoms.) S.H. Fu||Tibet, Sichuan, Qinghai||Hillside flow, alluvial plain, subalpine meadow, marshes, grassland||2400-5600|
|Ser. Yunnanenses (Frod.) S.H. Fu||R. yunnanesis (Franch.) S.H. Fu||Tibet, Sichuan||Rocks under forest, rocks beside ditches||2750-3200|
|R. henryi (Diels) S.H. Fu||Sichuan, Shanxi, Gansu||Slopes, beside ditches, rocks||1000-3300|
|Sect. Trifida (Frod.) S.H. Fu||–||R. sacra (Prain ex Hamet) S.H. Fu||Tibet, Qinghai||Rock crevices on mountain slopes, grassland on slopes||3500-4700|
Rhodiola is also known as “oriental god grass” and “plateau ginseng” and has very good value for medicine and healthy living. According to the basic tenets of traditional Chinese medicine, it can boost qi and dissipate blood stasis, unblock the blood vessels, relieve pain, fortify the spleen, treat palpitations, relieve coughing and shortness of breath, reduce fatigue and weakness. Rhodiola is also used as an anti-aging herb and for the treatment of aging-related diseases. Current pharmacological investigation reveals that Rhodiola has therapeutic value for many diseases such as Alzheimer’s disease (AD), Parkinson’s disease (PD), cerebrovascular disease, diabetes, and cardiovascular disease (CVD). The therapeutic actions and pharmacological functions of Rhodiola are listed in Table 2. Although Rhodiola is a large genus, only a few species have been investigated. In this review, the pharmacology of five species including R. rosea, R. crenulata, R. kirilowii, R. imbricata, and R. sachalinensis are discussed.
Pharmacological functions of medicinal Rhodiola on various ailments.
|Component or extraction method||Ailment||Pharmacological function||Refs|
|Salidroside||AD||Upregulates p-GSK-3β and downregulates p-tau||5|
|Upregulates PI3K/AKT signaling||6,7|
|Weakens the abnormal processing of APP||8|
|Induces antioxidant enzymes TRX, HO-1, and PRXI||9|
|Prevents caspase 3 activation, increases BAX/BCL-2 ratio, and reverses hippocampal neuronal loss||10|
|Protects mitochondria against sodium-azide-induced damage||11|
|Depression||Reduces TNF-α and IL-1ß levels||21|
|Attenuates levels of IL-6 and TNF-α||22|
|Attenuates NE and 5-HT levels in the prefrontal cortex||22|
|Regulates BDNF/TRKB signaling pathway||23|
|Huntington’s disease||Reduces neuronal death and behavioral dysfunction mediated by polyQ||32|
|Regulates AMPK/SIRT1/FOXO1 signaling||33|
|CVD||Attenuates H2O2-induced cell damage by downregulating Ca2+ and ROS via cAMP-dependent pathway||43|
|Promotes mitochondrial biogenesis and functions||44, 45|
|Increases the phosphorylation of AKT and ERK1/2; reduces the intracellular levels of ROS and the phosphorylation of JNK and p38 MAPK||47|
|Reduces the contents of CK, CK-MB, and LDH; increases GSH-Px and SOD activities; and reduces MDA content in liver tissue||48, 49|
|Increases levels of VEGF; upregulates HIF-1α protein expression and induces its translocation||49|
|Regulates BCL-2 protein family, reduces the expression of BAX; rescues the balance of pro- and anti-apoptotic proteins||50|
|Increases phosphorylation of AKT and reduces activation of caspase 3; markedly increases BCL-2/BAX ratio; preserves mitochondrial transmembrane potential||51|
|Diabetes||Reduces diabetes-induced oxidative stress||64|
|Inhibits the function and expression of CaL channels in vascular smooth muscle cells||67|
|Inhibits neuroinflammation and P2X7 receptor expression||68|
|Hepatic fibrosis||Inhibits lipid peroxidation||73|
|Acute liver fibrosis||Antioxidant activity and inhibits the function of HIF-1α||74|
|Bladder cancer||Inhibits the mTOR pathway and induces autophagy||79|
|Lung cancer||Reduces intracellular ROS generation and phosphor-p38 MAPK expression||80|
|Fibrosarcoma||Downregulates the ROS/PKC/ERK1/2 signaling pathway||81|
|Colon carcinoma||Inhibits the JAK2/STAT3-dependent pathway||82|
|Sarcoma||Reduces tumor-induced angiogenesis||83|
|Pulmonary hypertension||Regulates ET-1, NO, VEGF, ACE, NF-κB, TNF-α, and IL-6 expressions||59|
|Water extract of Rhodiola rosea||PD||Inhibits MAO-A and MAO-B activities and prevents the degradation of important neurotransmitters in PD patients||35|
|CVD||Reduces iNOS expression||55|
|Pulmonary hypertension||ACE-inhibitory activity||56|
|CVD||Causes withdrawal of sympathetic vasomotor tone and the circulatory angiotensin system||60|
|STZ-induced diabetes||Increases β-endorphin secretion from adrenal glands to activate opioid μ-receptors||72|
|Leukemia||Increases intracellular ROS in K-562 cell line; induces apoptosis, drives the cell to an oxidative-stress-induced cell death; arrests cell-cycle progression at G2/M||84|
|Ethanol extract of Rhodiola rosea||Pulmonary hypertension||ACE-inhibitory activity||56|
|Diabetes||Inhibits the activities of α-amylase, α-glucosidase, and ACE||56|
|Diabetic nephropathy||Lowers the expression of TGF-β1 in renal tissues||71|
|Polysaccharide from Rhodiola rosea||T lymphocytes in tumors||Increases the spleen and thymus indices and the production of cytokines (IL-2, TNF-α, and IFN-γ); increases the CD4+/CD8+ ratio||86|
|Tyrosol||Diabetes||Inhibits the activity of α-glucosidase||56|
|Oligomeric proanthocyanidin (OPCRR)||AD||Increases SOD and GSH-Px activities||19|
|Methanol extract of Rhodiola rosea||PD||Inhibits MAO-A and MAO-B activities and prevents the degradation of important neurotransmitters in PD patients||35|
|Ethanol extract of Rhodiola crenulata||Diabetes||Inhibits α-amylase, α-glucosidase, and ACE activities||56|
|Water extracts of Rhodiola crenulata||Diabetes||Inhibits α-amylase, α-glucosidase, and ACE activities||56|
|3% rosavin and 0.8% salidroside from Rhodiola rosea||Depression||Increases the blood-brain barrier permeability to precursors of DA and 5-HT;induces neural stem cell proliferation in the hippocampus||26|
|Rhodiola crenulata root extract||Hepatoma||Increases glycogen synthesis and the expression of regulatory enzymes in HepG2 cells; suppresses fat accumulation in hepatic cells under high-glucose conditions; is associated with the AMPK signaling pathway||77|
|Rhodiola crenulata root|
|Diabetes||Suppresses fructose-induced hyperinsulinemia and increases the insulin resistance index by modulating sarcolemmal and intracellular CD36 redistribution||69|
|Water extract of radix et rhizoma Rhodiola kirilowii||AMI||Elevates the expressions of HIF-1α, HIF-1β, and VEGF||59|
|Rhodiola rosea extract||CVD||Increases the levels of endogenous opioid peptides||54|
|Extract of Rhodiola rosea||Hypomnesia||Regulates the expression of monoamines and opioid peptides to increase the adaptability and activity of the central nervous system||40|
|Modulates the activity and levels of ACh in the brain||38|
|Increases the levels of NE, DA, 5-HT and ACh||39|
|Extract of Rhodiola rosea||PD||Facilitates production and proliferation of dopamine-producing cells||36|
Chemical constituents of Rhodiola
Rhodiola contains salidroside, flavonoids, terpenoids, sterols, tannins and many other compounds. These chemical constituents are the main focus for the investigation of Rhodiola biological activities. Since salidroside is the main bioactive constituent of Rhodiola and it is distributed in all parts of the plant and has various biological activities. This review focuses primarily on the application of salidroside and Rhodiola extract for the treatment of aging-related diseases.
Effects of Rhodiola on aging-related diseases
Effects of salidroside and Rhodiola extract on AD
Alzheimer’s disease is an age-related neurodegenerative disorder, which presents as learning and memory dysfunctions at an early stage and eventually evolves into cognitive disorder. The key risk factor for developing AD is age [1, 2].
The mechanisms of AD may be related to the deposition of β-amyloid (Aβ) peptide and intracellular neurofibrillary tangles consisting of hyperphosphorylated tau protein, which are important characteristics of AD and can lead to serial neuronal loss and brain atrophy [3,4].
Salidroside reduced neurodegeneration in tau-transgenic Drosophila and inhibited neuronal loss by upregulating phosphorylated GSK-3β (p-GSK-3β) and downregulating phosphorylated tau .
It also reduced Aβ levels and Aβ deposition in the brain by upregulating phosphatidylinositide 3-kinase (PI3K)/AKT signaling, thus reducing Aβ-induced cognitive impairment in rats [6,7]. In SH-SY5Y cells, salidroside also weakened the hypoxia-induced abnormal processing of amyloid precursor protein (APP), which is another risk factor for AD because abnormal APP generates significant Aβ .
Research has shown that oxidative stress plays an important role in the progression of AD . Salidroside protects neurons from oxidative stress by activating antioxidant enzymes thioredoxin (TRX), hemeoxygenase 1 (HO-1), and peroxiredoxin 1 (PRX1). Salidroside also reduces the expression of the proapoptotic protein BAX and increases the level of antiapoptotic protein BCL-XL . Neuronal apoptosis in the hippocampus is related to AD. Salidroside ameliorated the AD-associated cognitive deficit by preventing the activation of caspase 3, increasing the BAX/BCL-2 ratio, and reversing hippocampal neuronal loss caused by chronic cerebral hypoperfusion in rats. The study also showed that salidroside reduced apoptosis in the hippocampal CA1 area .
Disturbed energy metabolism is another main cause of AD thus, mitochondrial damage has been the focus of many AD studies. Salidroside greatly reduced cell damage and protected the mitochondria against damage induced by sodium azide , indicating that salidroside improves mitochondrial function.
The biotransformation of APP to Aβ is considered a very important step in the deposition of Aβ . Changes in APP metabolism plays a key role in the long-term action of acetylcholinesterase (AChE) inhibitors [3, 12], and the level of acetylcholine (ACh) in AD brain tissues is significantly reduced . Rhodiola rosea extracts (50 µg/ml) in different solvents (H2O, EtOAc, or BuOH) reduced AChE by 25.8%, 49.1%, and 40.8%, respectively. At a concentration of 100 µg/ml, extracts in the different solvents conferred 7.6%, 29.3%, and 24.3% neuroprotection, respectively.
These findings suggest that the Rhodiola rosea extract has potential therapeutic effects for AD, especially the EtOAc-based extract .
Recent studies have closely examined antioxidants as therapeutic strategies for patients with AD [15–18]. One active ingredient of Rhodiola rosea – oligomeric proanthocyanidin (OPCRR) – has demonstrated significant antioxidant activity. OPCRR significantly increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and reduced malondialdehyde (MDA) content in the serum, heart, liver, and brain tissues of mice, suggesting that OPCRR is a potent natural antioxidant that can be used for the treatment of AD .
Therefore, Rhodiola rosea may also be a potent antioxidant with potential therapeutic effects in patients with AD.
Effects of salidroside and Rhodiola extract on depression
Depression is a common mental health disorder characterized by sadness, loss of interest or pleasure, sense of guilt, feeling of shame, tiredness, disturbed sleep or appetite, and poor concentration. Research has demonstrated that salidroside may have antidepressant activity in mice .
Inflammation plays an important role in the development of depression. The serum levels of proinflammatory cytokines such as interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and IL-1β are elevated in major depressive disorders.
A study that evaluated the antidepressant effects of salidroside on olfactory-bulbectomized (OBX) rats showed that salidroside treatment significantly reduced TNF-α and IL-1β levels in the rat hippocampus .
Salidroside also significantly attenuated the levels of IL-6 and TNF-α in mice with depression-like behavior. 5-Hydroxytryptamine (5-HT) and norepinephrine (NE) in the brain are known to play important roles in the pathogenesis of depression; treatment with salidroside significantly attenuated NE and 5-HT levels in the prefrontal cortex in mice .
Brain-derived neurotrophic factor (BDNF), an important member of the neurotrophic protein family, is important in the pathophysiology of depression. Western blotting analysis showed that BDNF was increased in the rat hippocampus after treatment with salidroside, suggesting that salidroside plays a neuroprotective role by regulating the BDNF/TRKB signaling pathway .
The effects of Rhodiola rosea extract on depression were documented in a phase III randomized double-blind placebo controlled clinical trial. In the 6-week study, oral administration of Rhodiola rosea extract at a dose of 170 mg per day significantly reduced overall depressive symptoms including insomnia, emotional instability, and somatization, relative to those in the placebo-treated group .
The effects of an alcohol extract of Rhodiola rosea (usually containing 3% rosavin and 1% salidroside) on the central nervous systems of mice has been investigated. The extract displayed significant antidepressant-like activity in mice, which was independent of the dose .
A commercial Rhodiola rosea product (SHR-5, from Swedish Herbal Institute, Goteborg, Sweden, containing 3% rosavin and 0.8% salidroside) is considered to play a key role in stress modulation and the adaptability of the body.
Its mechanism may be related to its ability to increase the permeability of the blood-brain barrier to the precursors of dopamine (DA) and 5-HT .
A recent study indicated that depression is associated with suppressed cellular proliferation and apoptotic changes in hippocampal tissue . Rhodiola rosea extract improved 5-HT levels in the hippocampi of depressive rats, and low doses induced neural stem cell proliferation in their hippocampi, which may have repaired the injured hippocampal neurons .
Effects of salidroside and Rhodiola extract on other central nervous system diseases
Ischemic cerebrovascular disease is a serious malady often affecting the elderly . In a rat ischemia-reperfusion model of cerebrovascular disease, the antioxidative activity of salidroside significantly attenuated cerebral ischemic injury .
Polyglutamine (polyQ) aggregation plays a prominent role in the pathological process of Huntington’s disease. In a transgenic Caenorhabditis elegans model, salidroside reduced neuronal death and behavioral dysfunction mediated by polyQ toxicity .
Oxidative stress is known to be involved in status epilepticus; in a kainic-acid-induced model of status epilepticus, salidroside showed a neuroprotective effect by regulating AMPK/SIRT1/FOXO1 signaling .
PD is a neurodegenerative disease characterized by the progressive loss of dopaminergic neurons in the substantia nigra. Monoamine oxidase (MAO) is an important enzyme that modulates the metabolic recycling of catecholamines and 5-HT in the central nervous system [1, 34].
MAO-A and MAO-B are two important oxidases found at high levels in PD patients. Inhibition of these oxidases is currently used as a treatment for PD. Extracts of Rhodiola rosea contain both MAO-A and MAO-B inhibitors, which prevent the degradation of important neurotransmitters in PD patients .
Rhodiola rosea may also facilitate the production and proliferation of dopamine-producing cells .
Hypomnesia is a common symptom in the elderly. Oxidative damage and neuronal injury are two main causes of hypomnesia. In a randomized double-blind placebo-controlled clinical trial, an extract of Rhodiola rosea improved and enhanced the accuracy of memory 
. Rhodiola could also alleviate learning and memory impairments in rats with scopolamine-induced memory loss through its neuroprotective capability . Many studies have shown that Rhodiola rosea increased the levels of NE, DA, 5-HT and ACh, which stimulated central nervous system activity and improved learning and memory .
An extract of Rhodiola rosea showed a beneficial effect on learning and memory processes in mice with scopolamine-induced memory impairment possibly through modulation of brain ACh levels.
An extract of Rhodiola also improved the performance of rats in a water maze task and partly reversed the impairment of learning and memory induced by scopolamine .
These activities of Rhodiola rosea extract may be related to its regulation of the ACh, monoamines and opioid peptides, thereby increasing the adaptability and activity of the central nervous system . Therefore, Rhodiola rosea may effectively improve hypomnesia.
Effects of salidroside and Rhodiola extract on CVD
CVD is one of the most common diseases in the world and includes disorders of the heart and/or blood vessels such as coronary artery diseases, heart failure, heart arrhythmia. Although our understanding of CVD has improved, it is still the leading cause of death worldwide, especially in the elderly population . Two mechanisms known to be involved in ischemic heart disease are oxidative stress and cellular apoptosis. Many studies have demonstrated that salidroside protects against myocardial ischemia-reperfusion injury and myocardial hypoxic injury.
“Oxidative stress” refers to the imbalance between the oxidative and antioxidative systems in the body. The oxidative system includes reactive oxygen species (ROS) and reactive nitrogen species, whereas the antioxidative system includes various enzymes, such as SOD and GSH-Px .
Salidroside may attenuate hydrogen peroxide (H2O2)-induced cell damage by downregulating Ca2+ and ROS via a cAMP-dependent pathway . In a study using human umbilical vein endothelial cells (HUVECs), pretreatment with salidroside protected the cells from H2O2-induced injury by promoting mitochondrial biogenesis and its related functions [44, 45].
Using bone-marrow-derived endothelial progenitor cells (BM-EPCs), salidroside reduced the intracellular levels of ROS and increased H2O2-induced phosphorylation of AKT and ERK1/2 and simultaneously reduced H2O2-induced phosphorylation of JNK and p38 MAPK. Therefore, salidroside exerts protective effects against oxidative-stress-induced endothelial injury .
Antioxidant enzymes including SOD and GSH-Px are biocatalysts produced by living cells that can slow the rate of oxidation. MDA is the end-product of ROS-induced lipid peroxidation in organisms.
Creatine kinase (CK) activity is thought to be more reliable than electrocardiography in the diagnosis of myocardial infarction, and CK-MB, an isoenzyme of CK, has the highest diagnostic specificity.
Studies of the cardioprotective effects of salidroside showed that salidroside significantly reduced the levels of CK, CK-MB, and lactate dehydrogenase induced by exhaustive swimming. Salidroside also significantly increased GSH-Px and SOD activities and reduced the MDA content in rat liver tissue [47, 48], indicating that salidroside could protect the heart from repeated exhaustive injury.
Apoptotic cell death is an important mechanism of myocardial ischemia-reperfusion injury. Therefore, antiapoptotic strategies could be used to prevent reperfusion injury to the heart. Salidroside has been shown to regulate apoptosis in CVD. For example, a study by Zhang et al. revealed that salidroside significantly increases the levels of vascular endothelial growth factor (VEGF), upregulates the expression of hypoxia inducible factor 1α (HIF-1α) protein and induces its translocation.
These data indicate that salidroside exerts a protective effect against hypoxia-induced cardiomyocyte necrosis . Salidroside also reduces the expression of BAX and restores the balance of pro- and anti-apoptotic proteins. It protected endothelial cells from cobalt-chloride-induced apoptosis by regulating the expression of BCL-2 family proteins .
Furthermore, salidroside inhibited myocardial apoptosis in a rat model of acute myocardial infarction (AMI) and reduced the ischemia-mediated myocardial damage by increasing the phosphorylation of AKT and reducing the activation of caspase 3, markedly increasing the BCL-2/BAX ratio, and preserving the mitochondrial transmembrane potential . Salidroside also provided protection against coxsackievirus B3, which could cause viral myocarditis, both in vitro and in vivo [52, 53].
Rhodiola rosea extract, at a dose of 3.5 mg/kg given orally, prevented the reperfusion-induced loss of contraction strength in an isolated reperfused rat heart and prevented the reduction of coronary blood flow and the development of contracture in the post ischemic period. This effect is probably related to the increased levels of endogenous opioid peptides induced by Rhodiola rosea . The expressions of inducible nitric oxide synthase (iNOS) mRNA and protein increased markedly with myocardial ischemic injury while administration of a water extract of Rhodiola rosea downregulated the expression of iNOS in a dose-dependent manner. Therefore, the protective mechanism of Rhodiola rosea against ischemic injury to the heart may be through its downregulation of iNOS expression .
Pulmonary hypertension (PH) is a chronic, complex, and progressive disease that can lead to death. In general, Rhodiola species have an inhibitory effect on the development of hyperglycemia and hypertension. Both ethanol and water extracts of Rhodiola rosea showed strong angiotensin-converting enzyme (ACE)-inhibiting activities .
Rhodiola rosea is beneficial for high-altitude-related symptoms and the acute exacerbation of PH. The attenuation of PH by Rhodiola rosea has been demonstrated experimentally in chronically hypoxic rats.
These beneficial effects may be related to its possible regulation of signaling via several growth factors including endothelin 1, nitric oxide, VEGF, ACE, nuclear factor κB (NF-κB), TNF-α, and IL-6. Changes in these growth factors have been implicated in the pathogenesis of PH, which manifests as chronic pulmonary vasoconstriction, vasoproliferation, and vascular inflammation .
N-Tyrosol has an anti-arrhythmia activity, which could reduce the incidence of ventricular tachycardia and fibrillation, increase the percentage of animals without ventricular arrhythmia, and mitigate the severity of ventricular arrhythmia .
The rhizome of Rhodiola kirilowii significantly promoted the expression of the von Willebrand factor in the infarct and non-infarct zones of the rat myocardium. Rhodiola kirilowii also promoted angiogenesis in the myocardia of rats with AMI by elevating the expressions of HIF-1α, HIF-1β, and VEGF .
The systemic administration of the water-soluble fraction of Rhodiola rosea sacra radix induced potent hypotensive activity, which was mediated by the withdrawal of sympathetic vasomotor tone and the circulatory angiotensin system.
The positive inotropic and chronotropic effects of the water-soluble fraction may be attributable to its direct vagal inhibition in the heart .
Effects of salidroside and Rhodiola extract on diabetes
With the improvement of living standards, people live longer. This increases the prevalence of diabetes, which is the third most serious threat to human health after CVD and cancer . Traditional Chinese medicine is currently used in the treatment of diabetes and its associated complications. Recent studies have demonstrated that Rhodiola rosea extract and salidroside have potent effects on curbing diabetes.
Diabetes increases oxidative stress  by increasing the mitochondrial production of the superoxide anion, non-enzymatic glycation of proteins, and glucose autoxidation [63–65]. A previous study observed that salidroside administered daily to mice significantly reduced fasting blood glucose, total cholesterol, triglyceride, and MDA levels, while increasing serum insulin levels and SOD, GPx, and catalase activities.
These results indicate that salidroside protects against experimental diabetes, possibly through the reduction of diabetes-induced oxidative stress .
Vascular disease is regarded as a severe complication in diabetes mellitus. Hyperglycemia and hypertension are recognized as two of the leading risk factors for vascular complications in diabetic patients. When used in a combination therapy, salidroside simultaneously lowered blood glucose and blood pressure in diabetic rats. The mechanism involved the inhibition of the function and expression of L-type Ca2+ (CaL) channels in vascular smooth muscle cells .
Almost half of all diabetic patients also suffer from intractable neuropathic pain. Salidroside was reported to ameliorate diabetic neuropathic pain in rats by inhibiting neuroinflammation and the expression of P2X7 receptors . Insulin resistance is also a serious problem in diabetes and the Rhodiola rosea extract appears to affect insulin resistance.
The Rhodiola crenulata extract significantly suppressed fructose-induced hyperinsulinemia and increased the homeostasis model assessment of insulin resistance (HOMAR-IR) index and the adipose tissue insulin resistance index in male Sprague Dawley rats, indicating that the amelioration of insulin resistance by R. crenulata extract is attributable to its modulation of the sarcolemmal and intracellular redistribution of CD36 .
When an ethanol extract of Rhodiola rosea was administered orally to streptozocin (STZ)-induced diabetic rats, the extract showed a significant anti-hyperalgesic effect . Another study revealed that an ethanol extract of Rhodiola rosea protected diabetic rats from early nephropathy, which may have been associated with the reduced the expression of transforming growth factor β1 (TGF-β1) in renal tissues . A water extract of Rhodiola rosea administered orally to STZ-diabetic rats lowered plasma glucose and improved hyperglycemia, acting via an increase in β-endorphin secretion from the adrenal gland, which activated the opioid μ-receptors .
In another study, R. crenulata and Rhodiola rosea exerted significant inhibitory activities on α-amylase, α-glucosidase, and ACE. Furthermore, a major phenolic component of Rhodiola species, tyrosol, has a strong α-glucosidase-inhibitory activity, and may have utility as an effective therapy for postprandial hyperglycemia in patients with type II diabetes .
Taken together, Rhodiola species are potential pharmaceutical agents for the treatment of diabetes mellitus.
Effects of salidroside and Rhodiola extract on liver disease
Hepatic fibrosis is a common pathological basis for chronic liver disease and cirrhosis. Recent studies have demonstrated that the activation and proliferation of hepatic stellate cells (HSCs) are key factors in hepatic fibrogenesis, in which oxygen free radicals and lipid peroxidation play important roles. Salidroside has antioxidant activity, which significantly reduces the production of intracellular MDA and GSH, increases SOD and GSH-Px activities, markedly inhibits the proliferation of HSCs, and significantly lowers the levels of collagen in vitro. The mechanism of salidroside action on fibrosis may be related to its inhibition of lipid peroxidation .
In a mouse model of acute liver fibrosis induced with D-galactosamine and lipopolysaccharide, salidroside reduced elevated serum levels of aspartate amino transferase and alanine amino transferase as well as serum TNF-α and NO in a dose-dependent manner. It restored the depleted hepatic GSH, SOD, catalase and GSH-Px activities, and reduced MDA levels in the liver. Salidroside also reduced the degree of necrosis, caspase 3 and HIF-1α levels in the mouse liver. The hepatoprotective mechanism of salidroside may be related to its antioxidant activity and the functional inhibition of HIF-1α .
Oxidative stress is implicated in the functional impairment of adipose tissue and other tissues such as the liver. A Rhodiola extract protected the liver from oxidative-stress-induced damage while a methanolic extract of R. sachalinensis protected against D-galactosamine-induced cytotoxicity in primary mouse hepatocyte culture .
The liver is an important organ in the homeostasis of body energy because it regulates glucose and lipid metabolism, which are linked to the pathological progression of cirrhosis, non-alcoholic fatty liver disease, hepatitis, and liver cancer . Both in vitro and in vivo experiments have demonstrated that R. crenulata root extract increased glycogen synthesis and the expression of regulatory enzymes in HepG2 cells and suppressed fat accumulation in hepatic cells under high-glucose conditions. These results indicate that the regulatory effects of R. crenulata root extract on hepatic glycogen and lipid metabolism are associated with the AMPK signaling pathway .
Effects of salidroside and Rhodiola extract on cancer
The incidence of cancer increases markedly with age. People over the age of 65 years are generally more susceptible to cancer. The mechanism underlying this phenomenon may involve the accumulation of mutations in critical genes, which alter the normal programs of cell proliferation, differentiation, and death .
Salidroside selectively inhibited the growth of bladder cancer cell lines and TP53-defective cells, whereas it had little effect on non-malignant bladder epithelial cells. This may be attributable to its inhibition of the mTOR pathway and its induction of autophagy .
Oxidative stress is an important apoptotic stimulus in cancer cells and plays a critical role in both tumorigenesis and metastasis. Salidroside has potent antioxidant properties and could significantly inhibit the proliferation of A549 cells by inducing G0/G1 phase arrest and apoptosis. These activities could be related to its reduction of intracellular ROS generation and phosphor-p38 MAPK expression in A549 cells .
Another in vitro study demonstrated the inhibitory effects of salidroside on tumor cell metastasis in human fibrosarcoma HT1080 cells, mediated by the downregulation of the ROS/PKC/ERK1/2 signaling pathway . Salidroside also inhibited the migration and invasion on colon carcinoma SW1116 cells via the JAK2/STAT3-dependent pathway , and significantly reduced tumor-induced angiogenesis in mice .
An aqueous extract of the R. imbricata rhizome also reduces the proliferation of K-562 cells, increased intracellular ROS in the K-562 cell line, induced apoptosis and cell death. This extract also arrested cell-cycle progression at G2/M. These observations suggest that an aqueous extract of the R. imbricata rhizome has potent anticancer activities and might be useful in the treatment of leukemia .
The immune system plays an important role in the regulation and prevention of cancer . A polysaccharide from Rhodiola rosea is a promising candidate for reducing immune suppression in experimental tumors.
In an in vitro experiment, the polysaccharide had an acute cytotoxic effect on Sarcoma 180 (S-180) cells. In an in vivo experiment, it inhibited the growth of S-180 cells, dramatically enhanced immune responses, and protected the vital internal organs of S-180-bearing mice.
These activities were associated with increases in the spleen and thymus indices, the production of cytokines (IL-2, TNF-α, and interferon-γ), and the CD4+/CD8+ ratio in the tumor-bearing mice. These findings indicate that this polysaccharide from Rhodiola rosea is a novel and promising immune therapeutic agent for the treatment of cancer .
Traditional Chinese medicine offers a different perspective for the treatment of ailments and its role in anti-aging is garnering increasing attention [87–91]. Rhodiola is one of the most important and frequently used traditional Chinese herbal medicines. Among the chemical components of Rhodiola such as salidroside, polysaccharides, flavonoids, terpenoids, and many others, salidroside is the main bioactive agent.
Many extracts and single compounds isolated from Rhodiola have shown pharmacological effects on different organs and systems, including the cardiovascular and nervous systems. Rhodiola extracts and compounds display numerous bioactivities including anti-oxidative, anti-aging, anti-cancer and neuroprotective activities as well as possess significant adaptogenic properties.
Although Rhodiola is commonly used with other herbs in traditional Chinese medicine, many studies have indicated that this medicinal herb exerts significant pharmacological effects on its own.
The pharmacology of some traditional uses of Rhodiola has been validated in recent studies, although these studies were primarily conducted in vitro. Therefore, the effects of these compounds require verification in vivo.
Approximately 200 chemical compounds have been isolated from Rhodiola species, but only a few compounds have been investigated including salidroside, flavonoids, and polysaccharides.
The safety of these components in clinical practice must also be addressed. Unfortunately, there are only a few toxicological studies of these extracts and compounds, while in vitro and in vivo may not be applicable to humans. To explore the full medicinal potential of Rhodiola, the gaps between experimental results and clinical applications must be bridged by new innovations.
In conclusion, the evidence presented in this review strongly supports the proposition that Rhodiola has therapeutic properties for a variety of age-related diseases and therefore warrants further investigation.