AI-enabled eye scans could be used to rapidly predict whether a person is at high risk of heart disease


Artificial intelligence-enabled eye scans could be used to rapidly and accurately predict whether a person is at high risk of heart disease, a new study involving researchers from London’s Kingston University has established.

The findings could pave the way for cardiovascular screening to be done more quickly and simply by using cameras, without the need for blood tests or blood pressure measurements.

Circulatory diseases, including cardiovascular disease, coronary heart disease, heart failure and stroke, are major causes of ill health and death worldwide, currently accounting for one in four UK deaths alone. While several risk frameworks exist, these aren’t always able to accurately identify those who will go on to develop or die of circulatory diseases.

As part of the study, Kingston University Professor of Computer Vision Sarah Barman and postdoctoral researcher Roshan Welikala developed artificial intelligence (AI) algorithms that could reliably measure features on the retinal image, such as the width of blood vessels and how curved they were.

Working with colleagues from St George’s, University of London, the NIHR Biomedical Research Centre at Moorfields Eye Hospital and UCL Institute of Ophthalmology, as well as the MRC Epidemiology Unit at Cambridge University, they demonstrated this AI-enabled imaging could specify the risk of cardiovascular disease and stroke and act as an alternative predictive biomarker to traditional risk-scores for vascular health. The findings have now been published in the British Journal of Ophthalmology.

“Through this research we’ve shown an artificial intelligence eye scan that could be routinely carried out on the high street by an ophthalmologist is as good as a standard measure of cardiovascular risk,” Professor Barman said.

“Everyone who goes to the optician in the UK gets an eye scan and, as opposed to the standard methods that require a blood test from the GP, this type of screening would just need a retinal image and a few details, such as age, whether the patient smokes or not and some questions relating to their medical history.

“This method, which would allow wider screening of the population in a non-invasive way that could lead to early preventative treatments for those found to be at greater risk, has considerable potential.”

The researchers developed a fully automated AI-enabled algorithm, called QUARTZ, to assess the potential of retinal vasculature imaging alongside known risk factors to predict vascular health and death. The algorithm can assess a single retinal image in less than a minute.

Retinal images from 88,052 UK Biobank participants aged 40-69 were scanned using the algorithm, looking specifically at the width, vessel area and degree of curvature of the vessels to develop prediction models for stroke, heart attack and death from circulatory disease.

These models were then applied to the retinal images of 7,411 participants, aged 48-92, of the European Prospective Investigation into Cancer (EPIC)-Norfolk study.

The performance of QUARTZ was compared with the widely used Framingham Risk Scores framework. The health of participants was tracked for an average of seven to nine years, with a non-invasive risk score based on age, sex, smoking, medical history and retinal vasculature found to have performed as well as the Framingham framework.

A patient who has had a stroke on the left side. The top scans show the unaffected right eye; the visible lumps are soft drusen, lying underneath the RPE. The left retina (bottom scans) is packed with subretinal deposits on top of the RPE, caused by poor perfusion following the stroke. On the right, they create a wavy appearance in the layer above. The en face scan on the left has small, dark dots widely spread, with more toward the top of the image; those are the subretinal deposits. Note that the choroid layer has shrunk from 189 µm in the right eye to 90 µm in the left eye, a consequence of insufficient blood flow. ref link :

Heart, Eye and AI
Machine learning in cardiology

The role of AI and deep learning in cardiovascular applications is not new. Deep learning has been used in a number of cardiac imaging techniques, including intravascular OCT, echocardiography, cardiac magnetic resonance, computed tomography, and single-photon emission computed tomography [20].

In 2019, researchers developed a deep learning algorithm to predict mortality in hospitals after percutaneous coronary intervention based on age and ejection fraction, achieving up to 0.927 discrimination performance with the AdaBoost model [21]. A similar study used machine learning algorithms to predict diagnosis and disease complexity of over 10,000 patients with adult congenital heart disease or pulmonary hypertension; the algorithms reached an accuracy of 91.1% for diagnosis and 97.0% for disease complexity [22].

Retinal imaging, heart disease, and deep learning

The use of deep learning combined with retinal imaging in the diagnosis of cardiovascular conditions is a relatively new area of research (Table 1 [23-31]). In 2007, researchers from Australia, Singapore, and the USA showed that retinopathies obtained from fundus photographs were associated with the presence of any degree of coronary artery calcification (CAC) score > 0, measured by cardiac computed tomography scanning (odds ratio (OR): 1.22; 95% CI: 1.04 – 1.43) in a multi-ethnic population without clinical heart disease, after adjustment for multiple variables [23].

The same group of researchers also showed that narrower retinal arterioles obtained from fundus images were associated with concentric remodeling of the left ventricle as seen in cardiac magnetic resonance imaging (MRI) (OR: 2.06; 95% CI: 1.57 – 2.70) in a study with 4,593 individuals without any clinical cardiac disease [24].

Retinopathy was also correlated with left ventricular (LV) remodeling (OR: 1.31; 95% CI: 1.08 – 1.61), particularly in individuals with diabetes, hypertension or coronary calcification. The researchers inferred that microvascular disease, including retinopathies, can be reflective of subclinical macrovascular disease and share similar pathophysiological processes.

Table 1 – Studies Using Retinal Imaging to Predict Cardiovascular Disease Characteristics

StudyCountryNumberPopulationDeep learning involvementRetinal metric(s)CV outcomeResults
Cheung et al, 2007 [24]USA4,593Men and women aged 45 – 84 years without history of clinical CV diseaseNoneRetinal vascular caliber, retinopathyLeft ventricular concentric remodelingNarrower retinal arteriolar caliber (OR: 2.06) and retinopathy (OR: 1.31) associated with increased odds of concentric remodeling
Wong et al, 2008 [23]USA6,814Men and women aged 45 – 84 years without history of clinical CV diseaseNoneRetinopathy, retinal arteriovenous nicking, retinal arteriolar caliber, retinal venular caliberCACPresence of retinopathy was associated with presence of any CAC (OR: 1.22, 95% CI: 1.04 – 1.43); retinal arteriovenous nicking weakly associated with higher CAC scores; no significant associations of retinal arteriolar or venular caliber with CAC scores.
Kromer et al, 2018 [29]Germany106Males who experienced MI before age 50 and age-matched healthy malesNoneCentral retinal vessel caliber, AVRMINo significant differences in central retinal arterial/venous equivalent or AVR between MI group and control group.
Poplin et al, 2018 [28]UK, USA, and other countries in EyePACS database297,360Patients from UK Biobank and EyePACS databaseUsed model to predict CV risk factors from retinal fundus imagesRetinal fundus imagesCV risk factors, major adverse cardiovascular events (MACEs) within 5 yearsModel significantly predicted certain CV risk factors within given margin (P < 0.0001): age within 5 years at 78% accuracy; SBP within 15 mm Hg at 72% accuracy; DBP within 10 mm Hg at 79% accuracy; BMI within 5 at 80% accuracy. Model achieved AUC 0.70 (95% CI: 0.648 – 0.740) of predicting 5-year MACE from retinal fundus images alone compared to AUC 0.72 for European SCORE risk calculator.
Li et al, 2019 [27]China162Patients with ICA stenosisNoneSubfoveal choroidal thickness, choroidal vascular indexICA stenosisSubfoveal choroidal thickness (P < 0.05) and choroidal vascular index (P = 0.001) lower in severe ICA stenosis group.
Chang et al, 2020 [31]Korea38,824Participants who completed exams at HPC-SNUH between 2005 – 2016 and received retinal fundus examUsed model to predict atherosclerosis and risk of CV death relative to FRSRetinal fundus imagesPresence of atherosclerosis (DL-FAS), risk of CVD mortalityModel predicted atherosclerosis with accuracy of 58.3% and demonstrated significant association between DL-FAS and CVD mortality.
Dabrowska et al, 2020 [25]Poland12088 patients with essential hypertension and 32 healthy participants matched in age and genderNoneRetinal capillary flowArterial stiffness (measured by pulse wave velocity)Lower retinal capillary flow in patients with pulse wave velocity (PWV) > 10m/s compared to those with PWV ≤ 10 m/s (P = 0.02).
Druckenbrod et al, 2020 [26]USA143US veterans aged 29 – 91NoneSubfoveal choroidal thicknessCV disease risk factorsDiabetes associated with thinner choroid (P = 0.001). Hypertension (P = 0.006) and hyperlipidemia (P = 0.05) associated with thicker choroid.
Zhang et al, 2020 [30]China625Participants aged 24 – 83 yearsUsed model to predict CV risk factors from retinal fundus imagesRetinal fundus imagesHypertension, hyperglycemia, dyslipidemia, and other CV risk factorsModel predicted hypertension, hyperglycemia, and dyslipidemia with accuracies of 68.8%, 78.7%, and 66.7%, respectively.

MI: myocardial infarction; ICA: internal carotid artery; SBP: systolic blood pressure; DBP: diastolic blood pressure; BMI: body mass index; AUC: area under the ROC curve; CV: cardiovascular; CVD: cardiovascular disease; DL-FAS: deep learning-funduscopic atherosclerosis score; FRS: Framingham risk score; OR: odds ratio; CI: confidence interval; CAC: coronary artery calcification; AVR: arterial-venous ratio.

Another study tested the hypothesis that worsening hypertension was associated with capillary blood flow reduction and retinal microvessel remodeling, which was assessed by combining laser Doppler flowmetry with laser scanning tomography. The findings demonstrated that retinal capillary flow, and thus perfusion, was lower in patients with more advanced arterial stiffness (pulse wave velocity > 10 m/s) [25].

Cardiac microvascular disease can also be evaluated by assessing retinal arteriolar narrowing as detected by fundus photography. In patients without coronary artery calcification, narrower retinal arterioles were associated with lower hyperemic myocardial blood flow and perfusion reserve, which reflect microcirculation in the absence of stenosis [32].

Subfoveal choroidal thickness (SFCT) has been studied as a correlate to common cardiovascular disease (CVD) risk factors; multivariate analysis showed that diabetes was associated with thinner choroid (P = 0.001), whereas hypertension (P = 0.006) and hyperlipidemia (P = 0.05) were associated with thicker choroid [26]. In a separate study, SFCT was found to be significantly thicker in participants with hypercholesterolemia compared to those without (P = 0.041) [33]. However, in patients with severe internal carotid artery stenosis, SFCT was significantly lower. Those with severe stenosis also had a lower choroidal vascularity index (CVI), illustrating that CVI may be an indicator of stenotic changes in the internal carotid artery [27].

Serum amyloid A (SAA) protein deposition can lead to cardiac amyloidosis, although the incidence is very rare. The Beaver Dam Eye Study used retinal imaging to assess if there was a relationship between retinal vascular caliber and different inflammatory markers including SAA. SAA levels were higher in patients who had smaller arteriolar diameters after adjustment for other characteristics [34]. Stettler et al expanded on the Beaver Dam Eye Study and focused on SAA and retinal microvascular parameters in hypertensive patients with and without type 2 diabetes.

They found that SAA was significantly higher in diabetic patients compared to nondiabetics (3.15 mg/L vs. 2.65 mg/L; P = 0.03), and that diabetic patients had shorter retinal arteriolar vessels than nondiabetics (446.9 ± 103.7 vs. 466.4 ± 126.8 pixels; P = 0.03) [35]. Overall, more research is still needed to elucidate the association between retinal findings and cardiac amyloid.

In 2018, Google researchers trained a deep learning system based on AI to predict cardiovascular risk factors such as age, ethnicity, gender and smoking status in addition to systolic and diastolic blood pressure from retinal fundus images [28]. Researchers trained the system on images obtained from 284,335 patients of various ethnicities across two datasets, the UK Biobank and the EyePACS, and predicted results on 13,025 patients.

The AI system then combined the predicted information from all the risk factors to forecast the onset of major adverse cardiovascular events (MACEs) within 5 years. Using retinal fundus images alone, the model achieved an area under the ROC curve (AUC) of 0.70 (95% CI: 0.648 – 0.740) for predicting 5-year MACE values, which was comparable to an AUC of 0.72 (95% CI: 0.67 – 0.76) obtained from the European SCORE risk calculator. These results advocate for the use of machine learning to predict cardiovascular risk directly from retinal fundus images.

The ages of the participants from the above studies ranged from 40 to 85 years. Researchers are still exploring whether it is age or the characteristics of specific vascular disease that drives the learnability potential of the images. In a 2018 study, scientists compared features of the retinal microvasculature using OCT in a group of men, who experienced myocardial infarction before 50 years of age and found no significant differences in the arterial-venous ratio (AVR) or retinal vessel caliber when compared to an age-matched control group [29].

While the study was powered to 99% to detect the slightest differences between the two groups, no women were included in either group, suggesting that gender-specific hormonal influences could play a distinguishing role in the development of heart disease in men and women. Further analyses are being performed to examine risk stratification by gender for acute coronary syndromes [36].

In China, deep learning was utilized in a study of 625 subjects to predict hypertension, hyperglycemia, dyslipidemia, and other cardiovascular risk factors based on retinal fundus images. The model achieved an accuracy of 78.7% in detecting hyperglycemia, 68.8% in detecting hypertension, and 66.7% in detecting dyslipidemia.

Additionally, other risk factors such as age, drinking status, smoking status, salty taste, and body mass index (BMI) were also predicted with accuracies > 70%. These results support the application of deep learning to retinal fundus images in the identification of individuals at risk for CVD [30].

Furthermore, researchers validated a deep model using retinal fundus images not only to predict the presence of atherosclerosis, but to determine if the atherosclerosis score was correlated with cardiovascular death relative to the Framingham risk score. A total of 6,597 participants with retinal fundus exams and carotid artery sonography were used to develop the deep model for prediction of atherosclerosis, and 32,227 subjects with only retinal fundus exams were used to validate whether the atherosclerosis score could predict future cardiovascular death.

The model demonstrated an accuracy of 0.583 for predicting atherosclerosis, with a sensitivity of 0.891, but low specificity of 0.404. In terms of cardiovascular mortality, those with higher deep-learning funduscopic atherosclerosis scores (0.33 – 0.67 and 0.67- 1.00) had significantly higher risk of CVD mortality (hazard ratio (HR): 8.83; 95% CI: 1.41 – 6.15; and HR: 8.83; 95% CI: 3.16 – 24.7, respectively) than those with the lowest scores. Deep learning of retinal fundus images can be used to predict atherosclerosis, which can supplement current risk stratification scores for cardiovascular mortality [31].

Atherosclerosis contributes to the development of RVO, and hyperlipidemia was found to be much higher in those with RVO than in those without [37]. Matei et al evaluated statins for preventive efficacy in patients who were at high risk for developing RVO and for therapeutic efficacy in patients who had developed RVO. Unfortunately, neither a preventive nor therapeutic benefit was seen in patients who took statins compared to those who did not, although it was noted that the study was underpowered (n = 172 eyes) to detect a protective effect [38].

Other Applications of Retinal Microvasculature Analysis
Extensive research has demonstrated that there is a strong correlation between retinal vascular changes and clinical stroke [39]. Baseline retinopathy is associated with incidental stroke, and retinal venular widening is associated with an increased risk of stroke and stroke mortality. Furthermore, retinal vascular changes may differ based on various stroke subtypes and could help to discern clinical stroke from other causes of focal neurologic deficits. Interestingly, despite the strong correlation of retinal vascular changes with stroke, the addition of retinal imaging has only been shown to improve stroke risk stratification by about 10% from already established risk factors [39].

Alzheimer’s disease

Analysis of retinal microvasculature continues to expand as research finds that it reflects the state of health elsewhere in the body. In patients with Alzheimer’s disease, vessel density, perfusion density and GCIPLT in the central macula of the retina were found to be significantly reduced compared to both healthy controls and patients with mild cognitive impairment [40].

Another study found that Alzheimer’s patients with β-amyloid deposits in the brain as observed by positron emission tomography (PET) studies had texture differences in retinal microvasculature compared to control subjects without these deposits [41]. In Alzheimer’s patients, the retinal arterioles had bigger diameters near the optic nerve head while retinal venules showed an increased mean tortuosity. The amyloid deposits in the retina affecting the scattering of light in the hyperspectral retinal images are a potential biomarker for the disease.

Multiple sclerosis (MS)

Demyelinating lesions can lead to degeneration of optic nerve axons, which present as atrophied peripapillary retinal nerve fiber layer and ganglion cell-inner plexiform complex on OCT. OCT in MS patients can also show macular microcystoid changes that correlate to disease severity [42]. While use of OCT in MS has expanded considerably, additional research is needed to validate OCT as a biomarker in the diagnosis and progression of MS and as an indicator of response to therapy [43].

Chronic kidney disease (CKD)

The kidney and the eye share many structural and physiological similarities suggesting that an analysis of the eye may yield valuable information about renal function. Choroidal thinning was found to be directly correlated with a lower estimated glomerular filtration rate (eGFR) and higher proteinuria, but little is known about the exact mechanisms behind this association [44]. More studies are needed to explore the relationship between retinal imaging and CKD outcomes.

Photoacoustic imaging

Non-invasive and high resolution photoacoustic imaging has recently been adapted to optical applications to improve disease detection and potential treatment [45]. Scientists are now developing enhanced technologies to combine machine learning with quantitative photoacoustic imaging to measure parameters such as local blood oxygenation in real time [46]. Future studies are focusing on refining the programs to work on volumes of whole tissues at higher resolutions.

Biological image processing

Researchers at Google are now investigating the application of deep learning into extracting data from cellular images [47]. Scientists have built a software to detect morphology and localization of subcellular and nuclear organelles from fluorescence microscopy photos and to combine this information into a three-dimensional (3D) image [48]. This automated processing could dramatically improve the speed, efficiency, and objectivity of biological imaging analysis.

reference link :

More information: Alicja Regina Rudnicka et al, Artificial intelligence-enabled retinal vasculometry for prediction of circulatory mortality, myocardial infarction and stroke, British Journal of Ophthalmology (2022). DOI: 10.1136/bjo-2022-321842


Please enter your comment!
Please enter your name here

Questo sito usa Akismet per ridurre lo spam. Scopri come i tuoi dati vengono elaborati.