Fluorescence microscopy allows specific target detection down to the level of single molecules and has become an enabling tool in biological research.
To transduce the biological information to an imageable signal, we have developed a variety of fluorescent probes, such as organic dyes or fluorescent proteins with different colors.
Despite their success, a limitation on constructing small fluorescent probes is the lack of a general framework to achieve precise and programmable control of critical optical properties, such as color and brightness.
To address this challenge, we introduce metafluorophores, which are constructed as DNA nanostructure–based fluorescent probes with digitally tunable optical properties.
Each metafluorophore is composed of multiple organic fluorophores, organized in a spatially controlled fashion in a compact sub–100-nm architecture using a DNA nanostructure scaffold.
Using DNA origami with a size of 90 × 60 nm2, substantially smaller than the optical diffraction limit, we constructed small fluorescent probes with digitally tunable brightness, color, and photostability and demonstrated a palette of 124 virtual colors.
Using these probes as fluorescent barcodes, we implemented an assay for multiplexed quantification of nucleic acids.
Additionally, we demonstrated the triggered in situ self-assembly of fluorescent DNA nanostructures with prescribed brightness upon initial hybridization to a nucleic acid target.
Biomedical researchers are understanding the functions of molecules within the body’s cells in ever greater detail by increasing the resolution of their microscopes. However, what’s lagging behind is their ability to simultaneously visualize the many different molecules that mediate complex molecular processes in a single snap-shot.
Now, a team from Harvard’s Wyss Institute for Biologically Inspired Engineering, the LMU Munich, and the Max Planck Institute of Biochemistry in Germany, has engineered highly versatile metafluorophores by integrating commonly used small fluorescent probes into self-folding DNA structures where their colors and brightness can be digitally programmed.
This nanotechnological approach offers a palette of 124 virtual colors for microscopic imaging or other analytical methods that can be adapted in the future to visualize multiple molecular players at the same time with ultra-high definition.
The method is reported in Science Advances.
With their new method, the researchers address the problem that thus far only a limited number of molecular species can be visualized simultaneously with fluorescence microscopy in a biological or clinical sample.
By introducing fluorescent DNA nanostructures called metafluorophores — versatile fluorescent dyes whose colors are determined by how their individual components are arranged in 3-dimensional structures — they overcome this bottleneck.
“We use DNA nanostructures as molecular pegboards: by functionalizing specific component strands at defined positions of the DNA nanostructure with one of three different fluorescent dyes, we achieve a broad spectrum of up to 124 fluorescent signals with unique color compositions and intensities,” said Yin, who is a Core Faculty member at the Wyss Institute and Professor of Systems Biology at Harvard Medical School. “Our study provides a framework that allows researchers to construct a large collection of metafluorophores with digitally programmable optical properties that they can use to visualize multiple targets in the samples they are interested in.”
The DNA nanostructure-based approach can be used like a barcoding system to visually profile the presence of many specific DNA or RNA sequences in samples in what is called multiplexing.
To enable the visualization of multiple molecular structures in tissue samples whose thickness can limit the movement of larger DNA nanostructures and make it difficult for them to find their targets, and to reduce the possibility that they attach themselves to non-specific targets producing false fluorescence signals, the team took additional engineering steps.
“We developed a triggered version of our metafluorophore that dynamically self-assembles from small component strands that take on their prescribed shape only when they bind their target,” said Ralf Jungmann, Ph.D., who is faculty at the LMU Munich and the Max Planck Institute of Biochemistry and co-conducted the study together with Yin. “These in-situ assembled metafluorophores can not only be introduced into complex samples with similar combinatorial possibilities as the prefabricated ones to visualize DNA, but they could also be leveraged to label antibodies as widely used detection reagents for proteins and other biomolecules.”
“This new type of programmable, microscopy-enhancing DNA nanotechnology reveals how work in the Wyss Institute’s Molecular Robotics Initiative can invent new ways to solve long-standing problems in biology and medicine. These metafluorophores that can be programmed to self-assemble when they bind their target, and that have defined fluorescent barcode readouts, represent a new form of nanoscale devices that could help to reveal complex, multi-component, biological interactions that we know exist but have no way of studying today,” said Wyss Founding Director Donald Ingber, M.D., Ph.D., who is also the Judah Folkman Professor of Vascular Biology at Harvard Medical School and the Vascular Biology Program at Boston Children’s Hospital, and Professor of Bioengineering at the Harvard John A. Paulson School of Engineering and Applied Sciences.
Besides Yin and Jungmann, additional authors on the publication were Johannes Woehrstein and Maximilian Strauss, both members of Jungmann’s research group, and former members of the Yin group at the Wyss Institute, Luvena Ong, Ph.D., Bryan Wei, Ph.D. and David Zhang, Ph.D.