Researchers have identified a specific protein in the cell membranes of sperm that help them navigate their way to the egg for fertilization


Researchers have found that a protein in the cell membranes of sperm plays a key role in how they find their way to eggs.

The PMCA protein may also help explain how egg cells only interact with sperm from the same species.

PMCA may even be a target of drug discovery.

Sperm are excellent navigators.

If they weren’t we wouldn’t even be here.

Professor Manabu Yoshida from the Misaki Marine Biological Station at the University of Tokyo and colleagues investigate why sperm behave the way they do.

Sperm cells, bacteria and other microscopic organisms use varying concentrations of chemicals in their environment – concentration gradients – to approach or avoid something in a process called chemotaxis.

Egg cells release an attractant chemical, which lures the sperm.

The researchers studied this action in Ascidia – sea squirts, brainless tubular creatures, which are only mobile as larvae.

“We identified that a calcium transport protein – plasma membrane Ca2+ ATPase (PMCA) – has a key role in sperm chemotaxis,” says Yoshida.

“PMCA is abundant in the tails or flagella membranes of the ascidian sperm.

It binds to the species-specific attractant and alters how the flagella waves, thus directing movement of the sperm cell.”

This figure depicts how University of Tokyo researchers believe sperm cells navigate thanks to PMCA (plasma membrane Ca2+ ATPase) and SAAF (sperm activating and attracting factor). image is credited to Manabu Yoshida, The University of Tokyo.

The team used a range of techniques to measure the effect they observed.

These included a highly selective form of chromatography (separation of mixed compounds by diffusion in a fluid) called affinity column chromatography to isolate the attractant released by the egg; laser-based mass spectrometry, which uses lasers to identify what chemicals are in a sample; a quartz crystal microbalance, a sensitive microscopic weighing scale, to measure samples and how they change; and a high-speed camera to view sperm behavior in slow motion.

“With these methods we also found PMCA is responsible for regulating cellular calcium, whereas it was previously believed PMCA had no role in this,” continues Yoshida. “Now we know PMCA plays an important part in cellular function. It makes it a promising target for drug research.”


Funding: This work was supported by JSPS KAKENHI to MY (grant number 15H04398).

Source: Manabu Yoshida – University of Tokyo

Image Source:  credited to Manabu Yoshida, The University of Tokyo.
Original Research: Open access research for “Ca2+ efflux via plasma membrane Ca2+-ATPase mediates chemotaxis in ascidian sperm” by Kaoru Yoshida, Kogiku Shiba, Ayako Sakamoto, Jumpei Ikenaga, Shigeru Matsunaga, Kazuo Inaba & Manabu Yoshida in Scientific Reports. Published November 9 2018.


Please enter your comment!
Please enter your name here

Questo sito usa Akismet per ridurre lo spam. Scopri come i tuoi dati vengono elaborati.