The bone-building osteoblast cells have a complex relationship with cancer


In breast cancer, there are cases of women and men whose cancer returns in their bones 20-30 years after they were treated for their primary disease and thought they were cancer-free.

This phenomenon always puzzled Jefferson researcher Karen Bussard, Ph.D. How is it possible that breast cancer cells from a primary tumor are able to reach the bones when a patient is deemed “cancer-free” after treatment?

What was happening in bones that allowed the cancer cells to remain there for up to 30 years, alive but in a sleeping state, only to re-awaken decades later?

In a step towards answering these questions, Dr. Bussard recently discovered a type of bone cell that can subdue cancer cells, slowing their growth, even in one of the most aggressive types of breast cancer: triple negative.

The results, published in Breast Cancer Research, raise intriguing questions about how these bone cells exert their sleep-inducing influence, and whether it’s possible to replicate and permanently turn cancers dormant.

“Cancer has this uncanny ability to turn other cell types it comes in contact with to the cancer cell’s advantage,” says Dr. Bussard, Assistant Professor of Cancer Biology at Thomas Jefferson University and a researcher at the Sidney Kimmel Cancer Center – Jefferson Health.

“For example, cancer cells can turn the immune cells that should kill it, into its own guards.

However, we have now found a population of bone cells that not only resists, but subdues the cancer. It’s fascinating.”

Together with co-first authors and graduate students, Alexus D. Kolb and Alison B. Shupp and others, Dr. Bussard probed how bone cells change once they interact with breast cancer cells in the bone.

Specifically, they looked at osteoblasts – a kind of bone cell that lays down new bone, like cement, during growth and repair.

Bone is constantly being remodelled in a dynamic process where osteoblasts are responsible for bone formation and osteoclasts for its resorption.

Osteoblasts are specialized mesenchymal cells that undergo a process of maturation where genes like core-binding factor alpha1 (Cbfa1) and osterix (Osx) play a very important role.

Moreover, it was found recently that Wnt/ beta-catenin pathway plays a part on osteoblast differentiation and proliferation.

In fact, mutations on some of the proteins involved in this pathway, like the low-density lipoprotein receptor related protein 5/6 (LRP5/6) lead to bone diseases.

Osteoblast have also a role in regulation of bone resorption through receptor activator of nuclear factor-kappaB (RANK) ligand (RANKL), that links to its receptor, RANK, on the surface of pre-osteoblast cells, inducing their differentiation and fusion. On the other hand, osteoblasts secrete a soluble decoy receptor (osteoprotegerin, OPG) that blocks RANK/RANKL interaction by binding to RANKL and, thus, prevents osteoclast differentiation and activation.

Therefore, the balance between RANKL and OPG determines the formation and activity of osteoclasts.

Another factor that influences bone mass is leptin, a hormone produced by adipocytes that have a dual effect.

It can act through the central nervous system and diminish osteoblasts activity, or can have an osteogenic effect by binding directly to its receptors on the surface of osteoblast cells.

The research team showed that the osteoblast cells from mice as well as humans drastically changed their function after interacting with bone-metastatic breast cancer cells.

Earlier studies had shown that in advanced stage bone-metastatic breast cancer patients, osteoblasts stopped working; failing to produce a matrix that stabilizes and strengthens bone.

The changes lead to loss of bone density that is common in these patients.

In her new work, Dr. Bussard and colleagues showed that in earlier stages of the disease, when cancer cells first enter the bone, rather than producing new bone, osteoblasts may divert their energy toward producing factors to halt cancer cell growth.

When osteoblasts from humans or mice were exposed to triple negative or estrogen receptor positive breast cancer cells that had migrated to the bone, the osteoblasts released factors that changed cancer cell behavior.

These factors were able to swing the balance away from limitless cancer cell growth, and toward restoring production of the cell-cycle checkpoint protein p21, which stops metastatic breast cancer cells from replicating endlessly. Dr. Bussard’s team showed that cancer growth slowed in the presence of osteoblasts that had come in contact with metastatic breast cancer cells.

The osteoblasts that did not interact with metastatic breast cancer cells, on the other hand, were unable to slow cancer cell growth.

“The bone-building osteoblast cells have a complex relationship with cancer,” says Dr. Bussard.

“In advanced stages of the disease, we know that metastatic breast cancer cells can co-opt the normal cells of the bone to help cancer metastases thrive.

However, our new work suggests that during early stages of the disease, such as when metastatic breast cancer cells first migrate to the bone, these cancer-exposed osteoblasts resist and fight cancer growth.”

“Understanding how breast cancer cells prosper through metastasis to bone has been a long held goal of the breast cancer research community. Dr. Bussard’s breakthrough discoveries pave the way toward developing new strategies to prevent or treat metastatic disease”, says Karen E. Knudsen, Ph.D., EVP of Oncology Services and Enterprise Director of the Sidney Kimmel Cancer Center—Jefferson Health.

The next step, says Dr. Bussard, is to fully characterize the molecules that osteoblasts use to reign in cancer growth, and see whether it’s possible to turn that understanding toward treatments that can put cancer cells to sleep forever.

More information: Alexus D. Kolb et al, Osteoblasts are “educated” by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment, Breast Cancer Research (2019). DOI: 10.1186/s13058-019-1117-0
Journal information: Breast Cancer Research
Provided by Thomas Jefferson University


Please enter your comment!
Please enter your name here

Questo sito usa Akismet per ridurre lo spam. Scopri come i tuoi dati vengono elaborati.