Fractional anisotropy values are reduced in the corpus callosum and middle orbital gyrus in obese teens


Researchers using MRI have found signs of damage that may be related to inflammation in the brains of obese adolescents, according to a study being presented next week at the annual meeting of the Radiological Society of North America (RSNA).

Obesity in young people has become a significant public health problem.

In the U.S., the percentage of children and adolescents affected by obesity has more than tripled since the 1970s, according to the Centers for Disease Control and Prevention.

Data from the World Health Organization indicates that the number of overweight or obese infants and young children ages five years or younger increased from 32 million globally in 1990 to 41 million in 2016.

While obesity is primarily associated with weight gain, recent evidence suggests that the disease triggers inflammation in the nervous system that could damage important regions of the brain.

Developments in MRI like diffusion tensor imaging (DTI), a technique that tracks the diffusion of water along the brain’s signal-carrying white matter tracts, have enabled researchers to study this damage directly.

For the new study, researchers compared DTI results in 59 obese adolescents and 61 healthy adolescents, ages 12 to 16 years. From DTI, the researchers derived a measure called fractional anisotropy (FA), which correlates with the condition of the brain’s white matter. A reduction in FA is indicative of increasing damage in the white matter.

The results showed a reduction of FA values in the obese adolescents in regions located in the corpus callosum, a bundle of nerve fibers that connects the left and right hemispheres of the brain.

Decrease of FA was also found in the middle orbitofrontal gyrus, a brain region related to emotional control and the reward circuit. None of the brain regions in obese patients had increased FA.

“Brain changes found in obese adolescents related to important regions responsible for control of appetite, emotions and cognitive functions,” said study co-author Pamela Bertolazzi, a biomedical scientist and Ph.D. student from the University of São Paulo in Brazil.

This pattern of damage correlated with some inflammatory markers like leptin, a hormone made by fat cells that helps regulate energy levels and fat stores. In some obese people, the brain does not respond to leptin, causing them to keep eating despite adequate or excessive fat stores. This condition, known as leptin resistance, makes the fat cells produce even more leptin.

This shows brain scans from the study

Reduction in fractional anisotropy (FA) in obese patients compared to the control group: At the intersection of the alignment vectors, a large cluster of FA decrease located in the corpus callosum on the left. In red: Reduction of FA in obese patients compared to controls, and FA skeleton (green), superimposed on the mean of FA images in sample. The image is credited to Study author and RSNA.

Worsening condition of the white matter was also associated with levels of insulin, a hormone produced in the pancreas that helps regulate blood sugar levels. Obese people often suffer from insulin resistance, a state in which the body is resistant to the effects of the hormone.

“Our maps showed a positive correlation between brain changes and hormones such as leptin and insulin,” Dr. Bertolazzi said.

“Furthermore, we found a positive association with inflammatory markers, which leads us to believe in a process of neuroinflammation besides insulin and leptin resistance.”

Dr. Bertolazzi noted that additional studies are needed to determine if this inflammation in young people with obesity is a consequence of the structural changes in the brain.

“In the future, we would like to repeat brain MRI in these adolescents after multi-professional treatment for weight loss to assess if the brain changes are reversible or not,” she added.

Co-authors are Ricardo Uchida, D.Sc., Fabio L. Duran, D.Sc., Thaysa Neves, M.Sc., Elie Calfat, M.Sc., Naomi Costa, Estefania S. Fernandez, JoAnna D. Lima, Daniel A. Vasques, M.Sc., Cristiane Kochi, D.Sc., Marília Seelaender, D.Sc., Victor H. Otani, D.Sc., and Thais Z. Otani, D.Sc.

Magnetic resonance diffusion tensor imaging (DTI) provides information about water diffusion with the dominant direction parallel to the axonal orientation within the voxel of interest13. DTI enables the assessment of white and gray matter integrity in normal development and many disease states, by providing quantitative measures of fractional anisotropy (FA) and diffusivities, especially mean diffusivity (MD)49, that are biomarkers. DTI is usually acquired with phased array coils using parallel imaging methods to reduce image acquisition (ACQ) time and head motion artifacts. Using phased array coils, the signal-to-noise (SNR) within the acquired images is inhomogeneous with higher SNR peripherally and lower SNR centrally in the brain1012. The reproducibility, precision and accuracy of estimating tensor metrics are affected by SNR and analytic methodologies, such as operator-dependent manual region-of-interest (ROI) analysis for a single subject DTI data and operator-independent tract-based spatial statistics (TBSS)13 for analyzing multi-subject tensor data.

In the conventional data processing approaches, FA and MD values are calculated for each voxel, and maps are generated using whole brain automated analysis. The results are further assessed over ROIs defined by the operator. Bias-free measurements of FA require adequate SNR and regions with inherently lower FA require higher SNR1416. For regions of white matter which have high degrees of anisotropy such as genu and splenium of the corpus callosum, bias-free determination of FA is relatively easy to achieve. In a previous work14, on the other hand, it has been determined that bias free measurement of FA in the low FA region (putamen) at 1.5 T and 3 T requires at least number of signal average (NSA) = 9 and 6, respectively. In our institutes, three DTI scans are the maximum because patients also undergo other MRI scans. The diffusion scans are limited to less than 20 minutes. Diffusion data with NSA = 9 requires almost one hour to complete whole brain coverage, which is considered to be too long, and may not be practical for motion free scans in many study participants.

DTI of low FA regions of the brain is a challenge because of magnetic field strength limitation of MRI scanners and examination time restrictions of clinical examinations. In this study, we investigated an ROI-based tensor processing method in which image intensities inside an ROI with uniform diffusion properties are averaged first before calculating the diffusion tensor17,18. This method mitigates the requirement of long image acquisition times needed for bias-free FA and MD measurements and has not been applied to brain studies previously.

Media Contacts:
Linda Brooks – RSNA
Image Source:
The image is credited to Study author and RSNA.

Original Research: The study will be presented at the 105th Scientific Assembly and Annual Meeting of the Radiological Society of North America-RSNA 2019.


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