COVID-19: Silk performs similarly to surgical masks when used in conjunction with respirators

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“Cotton traps moisture like a sponge. But silk is breathable. It’s thinner than cotton and dries really fast,” Guerra said.

With COVID-19 surging in parts of the United States, face masks have become a focal point of prevention.

In the UC biology lab, researchers tested cotton and polyester fabric along with multiple types of silk to see how effective a barrier each is for repelling water, representing respiratory droplets containing the virus.

They found that silk worked far better as a moisture barrier than either polyester or cotton, both of which absorb water droplets quickly.

UC’s study concluded that silk performs similarly to surgical masks when used in conjunction with respirators but has the added advantages of being washable and repelling water, which would translate to helping to keep a person safer from the airborne virus.

“The ongoing hypothesis is that coronavirus is transmitted through respiratory droplets,” Guerra said. “If you wore layers of silk, it would prevent the droplets from penetrating and from being absorbed.

Recent work by other researchers also found that increasing layers of silk improves filtration efficiency. This means that silk material can repel and filter droplets. And this function improves with the number of layers.”

The study was published this month in the journal PLOS ONE.

Silk offers homemade solution for COVID-19 prevention
Postdoctoral researcher Adam Parlin holds up a styrofoam head draped with a silk mask. UC biologists examined how well face masks made from different materials repelled water or aerosolized droplets. Silk impeded the penetration and absorption of liquid and aerosolized droplets better than cotton or synthetic fabrics. Credit: Joseph Fuqua II/UC Creative + Brand

“We’re trying to address this critical problem. Health care workers still don’t have enough personal protective equipment, namely N95 respirators or basic surgical masks,” Guerra said.

Previously, Guerra studied the neurobiology behind the incredible multigenerational migration of monarch butterflies across North America. Now UC students raise silk moths (Bombyx mori) in Guerra’s biology lab.

UC postdoctoral researcher Adam Parlin wrote an undergraduate instruction manual for care and feeding of the moths he titled, “How to Train Your Bombyx,” a riff on the DreamWorks’ animated dragon movies.

The cover features a picture of the adult moth. With its big head, enormous eyes and fanned wings, the silk moth indeed resembles a night fury from the films.

“These little guys are entertaining,” he said.

As part of his research, Parlin studied how the caterpillars make their protective silk cocoons. When they reach a point in their life cycle, the caterpillars become manic workaholics.

For 72 straight hours they spin and spin their silk to create a luxurious, breathable fortress where they can pupate safely into a fuzzy white moth.

Researchers created cardboard arenas with a wooden dowel in the center upon which the caterpillars can spin their silk cocoons. The caterpillars work methodically and nonstop, initially spinning silk from the top of the dowel at an angle to the cardboard like a tent. Once the tent is finished, they work in earnest on building their grape-sized cocoon in a corner of it.

A time-lapse video shows a silkworm building a cocoon in 72 hours. Credit: Adam Parlin

“If the cocoon gets damaged, they just build a second layer around it,” Parlin said.

The moisture-trapping cocoon provides an ideal microclimate to keep the caterpillars happy despite any sudden changes in the weather.

“The silk cocoons prevent moisture from getting in and keeps the animal from desiccation or drying out,” Guerra said.

Now Guerra is investigating how long the virus survives on silk and other materials.

As shortages of personal protective equipment continue to plague health care providers, Guerra said homemade masks will continue to play an important role in keeping people safe from COVID-19.

“Silk has been with us for a while—since the days of the Silk Road,” Guerra said. “It’s not a new fabric, yet now we’re finding all these new uses for it.”


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The use of cloth masks, many of them homemade,1,2 has become widely prevalent in response to the 2019–2020 SARS-CoV-2 outbreak, where the virus can be transmitted via respiratory droplets.3−6 The use of such masks is also an anticipated response of the public in the face of future pandemics related to the respiratory tract.

However, there is limited data available today on the performance of common cloth materials used in such cloth masks,7−12 particularly their filtration efficiencies as a function of different aerosol sizes ranging from ∼10 nm to ∼10 μm scale sizes.

This is also of current significance as the relative effectiveness of different droplet sizes in transmitting the SARS-CoV-2 virus is not clear, and understanding the filtration response across a large bracketed size distribution is therefore important.13−16

In this paper, we report the results of experiments where we measure the filtration efficiencies of a number of common fabrics, as well as selective combinations for use as hybrid cloth masks, as a function of aerosol sizes ranging from ∼10 nm to 6 μm. These include cotton, the most widely used fabric in cloth masks, as well as fabric fibers that can be electrostatically charged, such as natural silk.

Respiratory droplets can be of various sizes17,18 and are commonly classified as aerosols (made of droplets that are <5 μm) and droplets that are greater than 5 μm.3 Although the fate of these droplets largely depends on environmental factors such as humidity, temperature, etc., in general, the larger droplets settle due to gravity and do not travel distances more than 1–2 m.19 However, aerosols remain suspended in the air for longer durations due to their small size and play a key role in spreading infection.14−16

The use of physical barriers such as respiratory masks can be highly effective in mitigating this spread via respiratory droplets.20−22 Filtration of aerosols follows five basic mechanisms: gravity sedimentation, inertial impaction, interception, diffusion, and electrostatic attraction.23,24

For aerosols larger than ∼1 μm to 10 μm, the first two mechanisms play a role, where ballistic energy or gravity forces are the primary influence on the large exhaled droplets. As the aerosol size decreases, diffusion by Brownian motion and mechanical interception of particles by the filter fibers is a predominant mechanism in the 100 nm to 1 μm range.

For nanometer-sized particles, which can easily slip between the openings in the network of filter fibers, electrostatic attraction predominates the removal of low mass particles which are attracted to and bind to the fibers. Electrostatic filters are generally most efficient at low velocities such as the velocity encountered by breathing through a face mask.25

There have been a few studies reported on the use of cloth face masks mainly during or after the Influenza Pandemic in 2009;8−12,26 However, there is still a lack of information that includes (i) the performance of various fabrics as a function of particle size from the nanoscale to the micron sized (particularly important because this covers the ∼10 nm to ∼5 μm size scale for aerosols) and (ii) the effect of hybrid multilayer approaches for masks that can combine the benefits of different filtering mechanisms across different aerosol size ranges.9,26

These have been the objectives of the experimental work described in this paper. In addition, we also point out the importance of fit (that leads to gaps) while using the face mask.27,28

The experimental apparatus (see Figure​Figure11) consists of an aerosol generation and mixing chamber and a downstream collection chamber. The air flows from the generation chamber to the collection chamber through the cloth sample that is mounted on a tube connecting the two chambers. The aerosol particles are generated using a commercial sodium chloride (NaCl) aerosol generator (TSI Particle Generator, model #8026), producing particles in the range of a few tens of nanometers to approximately 10 μm.

The NaCl aerosol based testing is widely used for testing face respirators in compliance with the NIOSH 42 CFR Part 84 test protocol.29,30 Two different particle analyzers are used to determine particle size dimensions and concentrations: a TSI Nanoscan SMPS nanoparticle sizer (Nanoscan, model #3910) and a TSI optical particle sizer (OPS, model #3330) for measurements in the range of 10 to 300 nm and 300 nm to 6 μm, respectively.

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Figure 1
Schematic of the experimental setup. A polydisperse NaCl aerosol is introduced into the mixing chamber, where it is mixed and passed through the material being tested (“test specimen”). The test specimen is held in place using a clamp for a better seal. The aerosol is sampled before (upstream, Cu) and after (downstream, Cd) it passes through the specimen. The pressure difference is measured using a manometer, and the aerosol flow velocity is measured using a velocity meter. We use two circular holes with a diameter of 0.635 cm to simulate the effect of gaps on the filtration efficiency. The sampled aerosols are analyzed using particle analyzers (OPS and Nanoscan), and the resultant particle concentrations are used to determine filter efficiencies.

Particles are generated upstream of the cloth sample, whose filtration properties are to be tested, and the air is drawn through the cloth using a blower fan which can be controlled in order to vary the airflow rate. Effective area of the cloth sample during the tests was ∼59 cm2. Measurements of particle size and distribution were made by sampling air at a distance of 7.5 cm upstream and 15 cm downstream of the cloth sample.

The differential pressures and air velocities were measured using a TSI digital manometer (model #AXD620) and a TSI Hot Wire anemometer (model #AVM410). The differential pressure (ΔP) across the sample material is an indicator of the comfort and breathability of the material when used as a face mask.31

Tests were carried out at two different airflows: 1.2 and 3.2 CFM, representative of respiration rates at rest (∼35 L/min) and during moderate exertion (∼90 L/min), respectively.32

The effect of gaps between the contour of the face and the mask as caused by an improper fit will affect the efficiency of any face mask.21,27,28,33 This is of particular relevance to cloth and surgical masks that are used by the public and which are generally not “fitted”, unlike N95 masks or elastomeric respirators.

A preliminary study of this effect was explored by drilling holes (symmetrically) in the connecting tube onto which the fabric (or a N95 or surgical mask) is mounted. The holes, in proximity to the sample (Figure​Figure11), resulted in openings of area ∼0.5−2% of the active sample area. This, therefore, represented “leakage” of the air around the mask.

Although the detailed transmission specifics of SARS-CoV-2 virus are not well understood yet, droplets that are below 5 μm are considered the primary source of transmission in a respiratory infection,13,15,34 and droplets that are smaller than 1 μm tend to stay in the environment as aerosols for longer durations of up to 8 h.19

Aerosol droplets containing the SARS-CoV-2 virus have been shown to remain suspended in air for ∼3 h.13,35 We have therefore targeted our experimental measurements in the important particle size range between ∼10 nm and 6 μm.

We tested the performance of over 15 natural and synthetic fabrics that included materials such as cotton with different thread counts, silk, flannel, and chiffon.

The complete list is provided in the Materials and Methods section. For comparison, we also tested a N95 respirator and surgical masks. Additionally, as appropriate, we tested the efficiency of multiple layers of a single fabric or a combination of multiple fabrics for hybrid cloth masks in order to explore combinations of physical filtering as well as electrostatic filtering.

Results and Discussion
We determine the filtration efficiency of a particular cloth as a function of particle size (Figure​Figure22) by measuring the concentration of the particles upstream, Cu (Figure​Figure22a,b) and the concentration of the particle downstream, Cd (Figure​Figure22c,d). Concentrations were measured in the size ranges of 10–178 nm (using the nanoscan tool) and 300 nm to 6 μm (using the optical particle sizer tool).

The representative example in Figure​Figure22 shows the case for a single layer of silk fabric, where the measurements of Cu and Cd were carried out at a flow rate of 1.2 CFM. Following the procedure detailed in the Materials and Methods section, we then estimated the filtration efficiency of a cloth from Cu and Cd as a function of aerosol particle size.

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Figure 2
Particle concentration as a function of particle size at a flow rate of 1.2 CFM. Plots showing the particle concentration (in arbitrary units) upstream and downstream through a single layer of natural silk for particle sizes <300 nm (a,c) and between 300 nm and 6 μm (b,d). Each bin shows the particle concentration for at least six trials. The particle concentrations in panels (b) and (d) are given in log scale for better representation of the data. The y-axis scales are the same for panels “a” and “c”; and for panels “b” and “d”.

The results plotted in Figure​Figure33a are the filtration efficiencies for cotton (the most common material used in cloth masks) with different thread counts (rated in threads per inch—TPI—and representative of the coarseness or fineness of the fabric).

We compare a moderate (80 TPI) thread count quilter’s cotton (often used in do-it-yourself masks) with a high (600 TPI) cotton fabric sample. Additionally, we also measured the transmission through a traditional cotton quilt where two 120 TPI quilter’s cotton sheets sandwich a ∼0.5 cm batting (90% cotton–5% polyester–5% other fibers).

Comparing the two cotton sheets with different thread counts, the 600 TPI cotton is clearly superior with >65% efficiency at <300 nm and >90% efficiency at >300 nm, which implies a tighter woven cotton fabric may be preferable.

In comparison, the single-layer 80 TPI cotton does not perform as well, with efficiencies varying from ∼5 to ∼55% depending on the particle size across the entire range. The quilt, a commonly available household material, with a fibrous cotton batting also provided excellent filtration across the range of particle sizes (>80% for <300 nm and >90% for >300 nm).

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Figure 3
Filtration efficiency of individual fabrics at a flow rate of 1.2 CFM (without gap). (a) Plot showing the filtration efficiencies of a cotton quilt consisting of two 120 threads per inch (TPI) cotton sheets enclosing a ∼0.5 cm thick cotton batting, 80 TPI quilters cotton (Q Cotton 80 TPI), and a 600 TPI cotton (cotton 600 TPI). (b) Plot showing the filtration efficiencies of one layer of natural silk (Silk-1L), four layers of natural silk (Silk-4L), one layer of flannel, and one layer of chiffon. The error bars on the <300 nm measurements are higher, particularly for samples with high filtration efficiencies because of the small number of particles generated in this size range, the relatively poorer counting efficiency of the detector at <300 nm particle size, and the very small counts downstream of the sample. The sizes of the error bars for some of the data points (>300 nm) are smaller than the symbol size and hence not clearly visible.

Electrostatic interactions are commonly observed in various natural and synthetic fabrics.36,37 For instance, polyester woven fabrics can retain more static charge compared to natural fibers or cotton due to their lower water adsorption properties.36 The electrostatic filtering of aerosols have been well studied.38

As a result, we investigated three fabrics expected to possess moderate electrostatic discharge value: natural silk, chiffon (polyester–Spandex), and flannel (cotton–polyester).36

The results for these are shown in Figure​Figure33b. In the case of silk, we made measurements through one, two, and four layers of the fabric as silk scarves are often wrapped in multiple layers around the face (the results for two layers of silk are presented in Figure S1 (Supporting Information) and omitted from this figure).

In all of these cases, the performance in filtering nanosized particles <300 nm is superior to performance in the 300 nm to 6 μm range and particularly effective below ∼30 nm, consistent with the expectations from the electrostatic effects of these materials. Increasing the number of layers (as shown for silk in Figure​Figure33b), as expected, improves the performance. We performed additional experiments to validate this using the 600 TPI cotton and chiffon (Figure S1).

We note that the performance of a four-layer silk composite offers >80% filtration efficiency across the entire range, from 10 nm to 6 μm.

In Figure​Figure44a, we combine the nanometer-sized aerosol effectiveness (for silk, chiffon, and flannel) and wearability (of silk and chiffon because of their sheer nature) with the overall high performance of the 600 TPI cotton to examine the filtration performance of hybrid approaches. We made measurements for three variations: combining one layer 600 TPI cotton with two layers of silk, two layers of chiffon, and one layer of flannel.

The results are also compared with the performance of a standard N95 mask. All three hybrid combinations performed well, exceeding 80% efficiency in the <300 nm range, and >90% in the >300 nm range. These cloth hybrids are slightly inferior to the N95 mask above 300 nm, but superior for particles smaller than 300 nm.

The N95 respirators are designed and engineered to capture more than 95% of the particles that are above 300 nm,39,40 and therefore, their underperformance in filtering particles below 300 nm is not surprising.

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Figure 4
Filtration efficiency of hybrid fabrics at a flow rate of 1.2 CFM. (a) Plot showing the filtration efficiencies without gap for an N95 respirator and a combination of different fabrics: 1 layer of 600 threads per inch (TPI) cotton and 2 layers of silk (cotton/silk), 1 layer of 600 TPI cotton and 2 layers of chiffon (cotton/chiffon), and 1 layer of 600 TPI cotton and 1 layer of flannel (cotton/flannel). (b) Plot showing the filtration efficiencies of a surgical mask and cotton/silk with (dashed) and without a gap (solid). The gap used is ∼1% of the active mask surface area. The error bars on the <300 nm measurements are higher, particularly for samples with high filtration efficiencies because of the small number of particles generated in this size range, the relatively poorer counting efficiency of the detector at <300 nm particle size, and the very small counts downstream of the sample. The sizes of the error bars for some of the data points (>300 nm) are smaller than the symbol size and hence not clearly visible.

It is important to note that in the realistic situation of masks worn on the face without elastomeric gasket fittings (such as the commonly available cloth and surgical masks), the presence of gaps between the mask and the facial contours will result in “leakage” reducing the effectiveness of the masks.

It is well recognized that the “fit” is a critical aspect of a high-performance mask.27,28,33,41 Earlier researchers have attempted to examine this qualitatively in cloth and other masks through feedback on “fit” from human trials.11,12 In our case, we have made a preliminary examination of this effect via the use of cross-drilled holes on the tube holding the mask material (see Figure​Figure11) that represents leakage of air.

For example, in Figure​Figure44b, we compare the performance of the surgical mask and the cotton/silk hybrid sample with and without a hole that represents about ∼1% of the mask area. Whereas the surgical mask provides moderate (>60%) and excellent (close to 100%) particle exclusion below and above 300 nm, respectively, the tests carried out with the 1% opening surprisingly resulted in significant drops in the mask efficiencies across the entire size range (60% drop in the >300 nm range).

In this case, the two holes were ∼0.635 cm in diameter and the mask area was ∼59 cm2. Similar trends in efficiency drops are seen in the cotton/silk hybrid sample, as well. Hole size also had an influence on the filtration efficiency. In the case of an N95 mask, increasing hole size from 0.5 to 2% of the cloth sample area reduced the weighted average filtration efficiency from ∼60 to 50% for a particle of size <300 nm.

It is unclear at this point whether specific aerodynamic effects exacerbate the “leakage” effects when simulated by holes. Its determination is outside the scope of this paper. However, our measurements at both the high flow (3.2 CFM) and low flow (1.2 CFM) rates show substantial drop in effectiveness when holes are present.

The results in Figures​Figures22–4 highlight materials with good performance. Several fabrics were tested that did not provide strong filtration protection (<30%), and examples include satin and synthetic silk (Table S1). The filtration efficiencies of all of the samples that we measured at both 1.2 CFM and 3.2 CFM are detailed in the Supporting Information (Figures S2–S4).

In Table 1, we summarize the key findings from the various fabrics and approaches that we find promising. Average filtration efficiencies (see Materials and Methods section for further detail) in the 10–178 nm and 300 nm to 6 μm range are presented along with the differential pressures measured across the cloths, which represents the breathability and degree of comfort of the masks.

The average differential pressure across all of the fabrics at a flow rate of 1.2 CFM was found to be 2.5 ± 0.4 Pa, indicating a low resistance and represent conditions for good breathability (Table 1).31 As expected, we observed an increase in the average differential pressures for the higher flow rate (3.2 CFM) case (Table S1).

Table 1

Filtration Efficiencies of Various Test Specimens at a Flow Rate of 1.2 CFM and the Corresponding Differential Pressure (ΔP) across the Specimena

 flow rate: 1.2 CFM
 filter efficiency (%)pressure differential
sample/fabric<300 nm average ± error>300 nm average ± errorΔP (Pa)
N95 (no gap)85 ± 1599.9 ± 0.12.2
N95 (with gap)34 ± 1512 ± 32.2
surgical mask (no gap)76 ± 2299.6 ± 0.12.5
surgical mask (with gap)50 ± 744 ± 32.5
cotton quilt96 ± 296.1 ± 0.32.7
quilter’s cotton (80 TPI), 1 layer9 ± 1314 ± 12.2
quilter’s cotton (80 TPI), 2 layers38 ± 1149 ± 32.5
flannel57 ± 844 ± 22.2
cotton (600 TPI), 1 layer79 ± 2398.4 ± 0.22.5
cotton (600 TPI), 2 layers82 ± 1999.5 ± 0.12.5
chiffon, 1 layer67 ± 1673 ± 22.7
chiffon, 2 layers83 ± 990 ± 13.0
natural silk, 1 layer54 ± 856 ± 22.5
natural silk, 2 layers65 ± 1065 ± 22.7
natural silk, 4 layers86 ± 588 ± 12.7
hybrid 1: cotton/chiffon97 ± 299.2 ± 0.23.0
hybrid 2: cotton/silk (no gap)94 ± 298.5 ± 0.23.0
hybrid 2: cotton/silk (gap)37 ± 732 ± 33.0
hybrid 3: cotton/flannel95 ± 296 ± 13.0
aThe filtration efficiencies are the weighted averages for each size range—less than 300 nm and more than 300 nm

Guidance
We highlight a few observations from our studies for cloth mask design:

Fabric with tight weaves and low porosity, such as those found in cotton sheets with high thread count, are preferable. For instance, a 600 TPI cotton performed better than an 80 TPI cotton. Fabrics that are porous should be avoided.

Materials such as natural silk, a chiffon weave (we tested a 90% polyester–10% Spandex fabric), and flannel (we tested a 65% cotton–35% polyester blend) can likely provide good electrostatic filtering of particles. We found that four layers of silk (as maybe the case for a wrapped scarf) provided good protection across the 10 nm to 6 μm range of particulates.

Combining layers to form hybrid masks, leveraging mechanical and electrostatic filtering may be an effective approach. This could include high thread count cotton combined with two layers of natural silk or chiffon, for instance. A quilt consisting of two layers of cotton sandwiching a cotton−polyester batting also worked well. In all of these cases, the filtration efficiency was >80% for <300 nm and >90% for >300 nm sized particles.

The filtration properties noted in (i) through (iii) pertain to the intrinsic properties of the mask material and do not take into account the effect of air leaks that arise due to improper “fit” of a mask on the user’s face. It is critically important that cloth mask designs also take into account the quality of this “fit” to minimize leakage of air between the mask and the contours of the face, while still allowing the exhaled air to be vented effectively.

Such leakage can significantly reduce mask effectiveness and are a reason why properly worn N95 masks and masks with elastomeric fittings work so well.

Conclusions
In conclusion, we have measured the filtration efficiencies of various commonly available fabrics for use as cloth masks in filtering particles in the significant (for aerosol-based virus transmission) size range of ∼10 nm to ∼6 μm and have presented filtration efficiency data as a function of aerosol particle size.

We find that cotton, natural silk, and chiffon can provide good protection, typically above 50% in the entire 10 nm to 6.0 μm range, provided they have a tight weave. Higher threads per inch cotton with tighter weaves resulted in better filtration efficiencies. For instance, a 600 TPI cotton sheet can provide average filtration efficiencies of 79 ± 23% (in the 10 nm to 300 nm range) and 98.4 ± 0.2% (in the 300 nm to 6 μm range).

A cotton quilt with batting provides 96 ± 2% (10 nm to 300 nm) and 96.1 ± 0.3% (300 nm to 6 μm). Likely the highly tangled fibrous nature of the batting aids in the superior performance at small particle sizes. Materials such as silk and chiffon are particularly effective (considering their sheerness) at excluding particles in the nanoscale regime (<∼100 nm), likely due to electrostatic effects that result in charge transfer with nanoscale aerosol particles.

A four-layer silk (used, for instance, as a scarf) was surprisingly effective with an average efficiency of >85% across the 10 nm −6 μm particle size range. As a result, we found that hybrid combinations of cloths such as high threads-per-inch cotton along with silk, chiffon, or flannel can provide broad filtration coverage across both the nanoscale (<300 nm) and micron scale (300 nm to 6 μm) range, likely due to the combined effects of electrostatic and physical filtering.

Finally, it is important to note that openings and gaps (such as those between the mask edge and the facial contours) can degrade the performance.

Our findings indicate that leakages around the mask area can degrade efficiencies by ∼50% or more, pointing out the importance of “fit”. Opportunities for future studies include cloth mask design for better “fit” and the role of factors such as humidity (arising from exhalation) and the role of repeated use and washing of cloth masks.

In summary, we find that the use of cloth masks can potentially provide significant protection against the transmission of particles in the aerosol size range.

References

  1. Ma N.; Jeffrey S. S. How to Sew a Fabric Face Mask. Science 2020, 367, 1424.10.1126/science.abb0736. [PubMed] [CrossRef] [Google Scholar]
  2. Centers for Disease Control and Prevention . Coronavirus Disease 2019 (COVID-19). Use of Cloth Face Coverings to Help Slow the Spread of COVID-19; CS316353B, available at https://www.cdc.gov/coronavirus/2019-ncov/prevent-getting-sick/diy-cloth-face-coverings.html, 2020; pp 1–3.
  3. Kutter J. S.; Spronken M. I.; Fraaij P. L.; Fouchier R. A.; Herfst S. Transmission Routes of Respiratory Viruses Among Humans. Curr. Opin. Virol. 2018, 28, 142–151. 10.1016/j.coviro.2018.01.001. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  4. Stelzer-Braid S.; Oliver B. G.; Blazey A. J.; Argent E.; Newsome T. P.; Rawlinson W. D.; Tovey E. R. Exhalation of Respiratory Viruses by Breathing, Coughing, and Talking. J. Med. Virol. 2009, 81, 1674–1679. 10.1002/jmv.21556. [PubMed] [CrossRef] [Google Scholar]
  5. Milton D. K.; Fabian M. P.; Cowling B. J.; Grantham M. L.; McDevitt J. J. Influenza Virus Aerosols in Human Exhaled Breath: Particle Size, Culturability, and Effect of Surgical Masks. PLoS Pathog. 2013, 9, e100320510.1371/journal.ppat.1003205. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  6. National Academies of Sciences . Medicine. Rapid Expert Consultation on the Possibility of Bioaerosol Spread of SARS-CoV-2 for the COVID-19 Pandemic; The National Academies Press: Washington, DC, 2020; p 3. [Google Scholar]
  7. National Academies of Sciences . Medicine. Rapid Expert Consultation on the Effectiveness of Fabric Masks for the COVID-19 Pandemic; The National Academies Press: Washington, DC, 2020; p 8. [Google Scholar]
  8. MacIntyre C. R.; Seale H.; Dung T. C.; Hien N. T.; Nga P. T.; Chughtai A. A.; Rahman B.; Dwyer D. E.; Wang Q. A Cluster Randomised Trial of Cloth Masks Compared With Medical Masks in Healthcare Workers. BMJ. Open 2015, 5, e00657710.1136/bmjopen-2014-006577. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  9. Shakya K. M.; Noyes A.; Kallin R.; Peltier R. E. Evaluating the Efficacy of Cloth Facemasks in Reducing Particulate Matter Exposure. J. Exposure Sci. Environ. Epidemiol. 2017, 27, 352–357. 10.1038/jes.2016.42. [PubMed] [CrossRef] [Google Scholar]
  10. Rengasamy S.; Eimer B.; Shaffer R. E. Simple Respiratory Protection–Evaluation of the Filtration Performance of Cloth Masks and Common Fabric Materials Against 20–1000 nm Size Particles. Ann. Occup. Hyg. 2010, 54, 789–798. 10.1093/annhyg/meq044. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  11. Davies A.; Thompson K. A.; Giri K.; Kafatos G.; Walker J.; Bennett A. Testing the Efficacy of Homemade Masks: Would They Protect in an Influenza Pandemic?. Disaster Med. Public Health Prep. 2013, 7, 413–418. 10.1017/dmp.2013.43. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  12. van der Sande M.; Teunis P.; Sabel R. Professional and Home-Made Face Masks Reduce Exposure to Respiratory Infections Among the General Population. PLoS One 2008, 3, e261810.1371/journal.pone.0002618. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  13. van Doremalen N.; Bushmaker T.; Morris D. H.; Holbrook M. G.; Gamble A.; Williamson B. N.; Tamin A.; Harcourt J. L.; Thornburg N. J.; Gerber S. I.; Lloyd-Smith J. O.; de Wit E.; Munster V. J. Aerosol and Surface Stability of SARS-CoV-2 as Compared With SARS-CoV-1. N. Engl. J. Med. 2020, 382, 1564.10.1056/NEJMc2004973. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  14. Morawska L.; Cao J. Airborne Transmission of SARS-Cov-2: The World Should Face the Reality. Environ. Int. 2020, 139, 105730.10.1016/j.envint.2020.105730. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  15. Wang J.; Du G. COVID-19 May Transmit Through Aerosol. Ir. J. Med. Sci. 2020, 1–2. 10.1007/s11845-020-02218-2. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  16. Santarpia J. L.; Rivera D. N.; Herrera V.; Morwitzer M. J.; Creager H.; Santarpia G. W.; Crown K. K.; Brett-Major D.; Schnaubelt E.; Broadhurst M. J.; Lawler J. V.; Reid S. P.; Lowe J. J.. Transmission Potential of SARS-CoV-2 in Viral Shedding Observed at the University of Nebraska Medical Center. 2020, medRxiv; https://10.1101/2020.03.23.20039446 (accessed 2020-04-04).
  17. Zhang H.; Li D.; Xie L.; Xiao Y. Documentary Research of Human Respiratory Droplet Characteristics. Procedia Eng. 2015, 121, 1365–1374. 10.1016/j.proeng.2015.09.023. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  18. World Health Organization . Annex C – Respiratory droplets. In Natural Ventilation for Infection Control in Health-Care Settings; Atkinson J., Chartier Y., Pessoa-Silva C. L., Jensen P., Li Y., Seto W. H., Eds.; World Health Organization: Geneva, 2009; pp 77–82. [Google Scholar]
  19. Morawska L. Droplet Fate in Indoor Environments, or Can We Prevent the Spread of Infection?. Indoor Air 2006, 16, 335–347. 10.1111/j.1600-0668.2006.00432.x. [PubMed] [CrossRef] [Google Scholar]
  20. Ching W.-H.; Leung M. K. H.; Leung D. Y. C.; Li Y.; Yuen P. L. Reducing Risk of Airborne Transmitted Infection in Hospitals by Use of Hospital Curtains. Indoor Built Environ. 2008, 17, 252–259. 10.1177/1420326X08091957. [CrossRef] [Google Scholar]
  21. Lai A. C. K.; Poon C. K. M.; Cheung A. C. T. Effectiveness of Facemasks to Reduce Exposure Hazards for Airborne Infections Among General Populations. J. R. Soc., Interface 2012, 9, 938–948. 10.1098/rsif.2011.0537. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  22. Leung N. H. L.; Chu D. K. W.; Shiu E. Y. C.; Chan K.-H.; McDevitt J. J.; Hau B. J. P.; Yen H.-L.; Li Y.; Ip D. K. M.; Peiris J. S. M.; Seto W.-H.; Leung G. M.; Milton D. K.; Cowling B. J. Respiratory Virus Shedding in Exhaled Breath and Efficacy of Face Masks. Nat. Med. 2020, 10.1038/s41591-020-0843-2. [CrossRef] [Google Scholar]
  23. Hinds W. C.9 – Filtration. In Aerosol Technology: Properties, Behavior, and Measurement of Airborne Particles, 2nd ed.; John Wiley & Sons: New York, 1999; pp 182–205. [Google Scholar]
  24. Vincent J. H.21 – Aerosol Sample Applications and Field Studies. In Aerosol Sampling. Science, Standards, Instrumentation and Applications; Vincent J. H., Ed.; John Wiley & Sons: New York, 2007; pp 528–529. [Google Scholar]
  25. Colbeck I.; Lazaridis M.. 5 – Filtration Mechanisms. In Aerosol Science: Technology and Applications, 1st ed.; Colbeck I., Lazaridis M., Eds.; John Wiley & Sons: New York, 2014; pp 89–118. [Google Scholar]
  26. Jung H.; Kim J.; Lee S.; Lee J.; Kim J.; Tsai P.; Yoon C. Comparison of Filtration Efficiency and Pressure Drop in Anti-Yellow Sand Masks, Quarantine Masks, Medical Masks, General Masks, and Handkerchiefs. Aerosol Air Qual. Res. 2014, 14, 991–1002. 10.4209/aaqr.2013.06.0201. [CrossRef] [Google Scholar]
  27. Holton P. M.; Tackett D. L.; Willeke K. Particle Size-Dependent Leakage and Losses of Aerosols in Respirators. Am. Ind. Hyg. Assoc. J. 1987, 48, 848–854. 10.1080/15298668791385697. [PubMed] [CrossRef] [Google Scholar]
  28. Rengasamy S.; Eimer B. C. Nanoparticle Penetration Through Filter Media and Leakage Through Face Seal Interface of N95 Filtering Facepiece Respirators. Ann. Occup. Hyg. 2012, 56, 568–580. 10.1093/annhyg/mer122. [PubMed] [CrossRef] [Google Scholar]
  29. Rengasamy S.; Zhuang Z.; Niezgoda G.; Walbert G.; Lawrence R.; Boutin B.; Hudnall J.; Monaghan W. P.; Bergman M.; Miller C.; Harris J.; Coffey C. A Comparison of Total Inward Leakage Measured Using Sodium Chloride (NaCl) and Corn Oil Aerosol Methods for Air-Purifying Respirators. J. Occup. Environ. Hyg. 2018, 15, 616–627. 10.1080/15459624.2018.1479064. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  30. Electronic Code of Federal Regulations (eCFR), Title 42: Public Health, Part 84—Approval of Respiratory Protective Devices. Code of Federal Regulations, April 2020.
  31. Lord J. 35—The Determination of the Air Permeability of Fabrics. J. Text. I. 1959, 50, T569–T582. 10.1080/19447025908659937. [CrossRef] [Google Scholar]
  32. Silverman L.; Lee G.; Plotkin T.; Sawyers L. A.; Yancey A. R. Air Flow Measurements on Human Subjects With and Without Respiratory Resistance at Several Work Rates. AMA Arch. Ind. Hyg. Occup. Med. 1951, 3, 461–478. [PubMed] [Google Scholar]
  33. Grinshpun S. A.; Haruta H.; Eninger R. M.; Reponen T.; McKay R. T.; Lee S.-A. Performance of an N95 Filtering Facepiece Particulate Respirator and a Surgical Mask During Human Breathing: Two Pathways for Particle Penetration. J. Occup. Environ. Hyg. 2009, 6, 593–603. 10.1080/15459620903120086. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  34. Wells W. F. Airborne Contagion and Air Hygiene: An Ecological Study of Droplet Infections. J. Am. Med. Assoc. 1955, 159, 90.10.1001/jama.1955.02960180092033. [CrossRef] [Google Scholar]
  35. Huang H.; Fan C.; Li M.; Nie H.-L.; Wang F.-B.; Wang H.; Wang R.; Xia J.; Zheng X.; Zuo X.; Huang J. COVID-19: A Call for Physical Scientists and Engineers. ACS Nano 2020, 10.1021/acsnano.0c02618. [PMC free article] [PubMed] [CrossRef] [Google Scholar]
  36. Perumalraj R. Characterization of Electrostatic Discharge Properties of Woven Fabrics. J. Textile Sci. Eng. 2015, 06, 1000235.10.4172/2165-8064.1000235. [CrossRef] [Google Scholar]
  37. Frederick E. R. Fibers, Filtration and Electrostatics – A Review of the New Technology. J. Air Pollut. Control Assoc. 1986, 36, 205–209. 10.1080/00022470.1986.10466060. [CrossRef] [Google Scholar]
  38. Sanchez A. L.; Hubbard J. A.; Dellinger J. G.; Servantes B. L. Experimental Study of Electrostatic Aerosol Filtration at Moderate Filter Face Velocity. Aerosol Sci. Technol. 2013, 47, 606–615. 10.1080/02786826.2013.778384. [CrossRef] [Google Scholar]
  39. Bałazy A.; Toivola M.; Adhikari A.; Sivasubramani S. K.; Reponen T.; Grinshpun S. A. Do N95 Respirators Provide 95% Protection Level Against Airborne Viruses, and How Adequate are Surgical Masks?. Am. J. Infect. Control 2006, 34, 51–57. 10.1016/j.ajic.2005.08.018. [PubMed] [CrossRef] [Google Scholar]
  40. Balazy A.; Toivola M.; Reponen T.; Podgorski A.; Zimmer A.; Grinshpun S. A. Manikin-Based Performance Evaluation of N95 Filtering-Facepiece Respirators Challenged With Nanoparticles. Ann. Occup. Hyg. 2005, 50, 259–269. [PubMed] [Google Scholar]
  41. National Academies of Sciences . Medicine. Reusable Elastomeric Respirators in Health Care: Considerations for Routine and Surge Use; The National Academies Press: Washington, DC, 2019; p 226. [Google Scholar]
  42. Bullock W. H.; Ignacio J. S.. A Strategy for Assessing and Managing Occupational Exposures; AIHA Press, American Industrial Hygiene Association, 2006. [Google Scholar]

More information: Adam F. Parlin et al, A laboratory-based study examining the properties of silk fabric to evaluate its potential as a protective barrier for personal protective equipment and as a functional material for face coverings during the COVID-19 pandemic, PLOS ONE (2020). DOI: 10.1371/journal.pone.0239531

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