Mouthwash Chlorhexidine and Listerine disrupt the COVID-19 virus within seconds

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Researchers at Rutgers School of Dental Medicine have found evidence that two types of mouthwash disrupt the COVID-19 virus under laboratory conditions, preventing it from replicating in a human cell.

The study, published in the journal Pathogens, found that Listerine and the prescription mouthwash Chlorhexidine disrupted the virus within seconds after being diluted to concentrations that would mimic actual use. Further studies are needed to test real-life efficacy in humans.

The study was conducted in a lab using concentrations of the mouthwash and the time it would take to contact tissues to replicate conditions found in the mouth, said Daniel H. Fine, the paper’s senior author and chair of the school’s Department of Oral Biology.

The study found two other mouthwashes showed promise in potentially providing some protection in preventing viral transmission: Betadine, which contains povidone iodine, and Peroxal, which contains hydrogen peroxide.

However, only Listerine and Chlorhexidine disrupted the virus with little impact on skin cells inside the mouth that provide a protective barrier against the virus.

“Both Povidone iodine and Peroxal caused significant skin cell death in our studies, while both Listerine and Chlorhexidine had minimal skin cell killing at concentrations that simulated what would be found in daily use,” said Fine.

The team studied the efficacy of mouthwash potential for preventing viral transmission to better understand how dental providers can be protected from aerosols exhaled by patients.

“As dentists, we’re right there in a patient’s face. We wanted to know if there’s something that might lower the viral load,” said coauthor Eileen Hoskin, an assistant professor at Rutgers School of Dental Medicine.

Fine cautions the public against relying on mouthwash as a way to slow the spread until it is proven in clinical trials on humans.

“The ultimate goal would be to determine whether rinsing two or three times a day with an antiseptic agent with active anti-viral activity would have the potential to reduce the ability to transmit the disease. But this needs to be investigated in a real-world situation,” he said.

Previous research has shown various types of antiseptic mouthwashes can disrupt the novel coronavirus and temporarily prevent transmission, but this was one of the first studies that examined antiseptic rinse concentrations, time of contact and the skin-cell killing properties that simulated oral conditions.

The study was conducted by a team of dental school scientists and virologist at the Public Health Research Institute.

Since the SARS CoV-2 virus responsible for COVID-19 enters primarily through the oral and nasal cavity, oral biologists should be included in these studies because they have an in-depth understanding of oral infectious diseases,” said Fine.


Antiseptic mouthwashes have been widely used as a standard measure before routine dental treatment, especially preoperatively.1, 2 They have an essential role in reducing the number of microorganisms in the oral cavity.3 Recent publications have suggested that rinsing the oral cavity may control and reduce the risk of transmission of SARS-CoV-2.4, 5 However, specific evidence for the safety and efficacy of the use of antiseptic mouthwashes in COVID-19 positive patients is lacking and unclear, so this paper aims to provide a comprehensive review of the current recommendations on the use of mouthwashes against the COVID-19 pandemic and to analyse the advantages and disadvantages of most conventional antiseptic mouthwashes used in dentistry.

Pathogenesis of coronavirus disease 2019

Coronaviruses are a group of enveloped RNA viruses that present a typical structure with the “spike protein” in its membrane envelope.6, 7 The interaction between this protein and angiotensin-converting enzyme 2 (ACE2) receptors is responsible for the entry of the virus into cells.8 The distribution of ACE2 receptors in different parts of the body may indicate possible routes of infection.9, 10

The membrane bound to ACE2 is found in different tissue cells, including mucosal tissues, gingiva, non-keratinising squamous epithelium, and epithelial cells of the tongue and salivary glands.8, 11 A high SARS-CoV-2 viral load has also been detected in saliva,12 and it its presence has even been suggested in periodontal pockets.13

These findings agree with previous investigations that have suggested that virus transmission can be closely connected with saliva interactions14, 15 making oral tissues a possible reservoir from which SARS-CoV-2 transmission may occur during coughing, sneezing, talking, and even during dental care.4, 12, 16

Oral antiseptics used against viral infections

Mouthwashes are widely used solutions for rinsing the mouth, especially before oral surgery, due to their ability to reduce the number of microorganisms in the oral cavity1, 2 and colony-forming units in dental aerosols.3 Although there is still no clinical evidence that the use of mouthwashes could prevent SARS-CoV-2 transmission, the American Dental Association (ADA)17 and the Center for Disease Control and Prevention (CDC)18 have recommended the use of preprocedural mouthwashes before oral procedures.

Chlorhexidine (CHX)

CHX is a broad-spectrum antiseptic that acts against Gram-positive and Gram-negative bacteria, aerobes, facultative anaerobes, and fungus by increasing the permeability of the bacterial cell wall, causing its lysis.19, 20 It is used in dentistry to reduce dental plaque and treat periodontal disease.21

Evidence indicates an in vitro effect against lipid-enveloped viruses such as influenza A, parainfluenza, herpes virus 1, cytomegalovirus, and hepatitis B.22 Although COVID-19 is an enveloped virus, 0.12% CHX gluconate was suggested to have little or no effect against coronaviruses when compared with other mouthwashes.4, 23, 24 However, Yoon et al6 found SARS-CoV-2 suppression for two hours after using 15 ml 0.12% CHX once, suggesting that its use would be beneficial for the control of COVID-19 transmission.

Hydrogen peroxide (H2O2)

H2O2 has been used in dentistry alone or combined with salts since the start of the century.25 As a mouthwash, it is an odourless, clear, and colourless liquid.26 Lack of an adverse soft tissue effect was found in many studies of 1%–1.5% H2O2 used as a daily rinse over two years’ follow-up.27, 28

An in vitro study found that 3% H2O2 effectively inactivated adenovirus types 3 and 6, adeno-associated virus type 4, rhinoviruses 1A, 1B, and type 7, myxoviruses, influenza A and B, respiratory syncytial virus, strain long, and coronavirus strain 229E within 1–30 minutes, discovering that coronaviruses and influenza viruses were the most sensitive.29 Since SARS-CoV2 is vulnerable to oxidation, preprocedural mouthrinses containing oxidative agents such as 1% H2O2 have been suggested to reduce the salivary viral load.4, 17

Cetylpyridinium chloride (CPC)

CPC is a quaternary ammonium compound that is safe for use in humans.30, 31 CPC 0.05% has been used to reduce dental plaque and gingivitis32 as an alternative in patients who develop mucosal irritation and stains related to CHX.33 The antiviral effect of CPC has been demonstrated in influenza patients, significantly reducing the duration and severity of cough and sore throat.31, 34 Hypotheses about a possible action over SARS-CoV-2 are based on its lysosomotropic mechanism of action and its ability to destroy viral capsids.35 These findings indicate that CPC could be effective against other enveloped viruses such as coronaviruses.

Iodopovidone

Povidone-iodine (PVP-I) is a water-soluble iodine complex that has been widely used as a pre-surgical skin antiseptic and as a mouthwash.36 It is typically used in a 1% concentration37 for mucositis, prophylaxis of oropharyngeal infections, and prevention of ventilator-associated pneumonia.

Its antimicrobial action occurs after free iodine dissociates from polyvinylpyrrolidone, then iodine rapidly penetrates microbes to disrupt proteins and oxidises nucleic acid structures causing microbial death.38, 39 Previously studies have shown that PVP-I has higher virucidal activity than other commonly used antiseptic agents, including CHX and benzalkonium chloride.40

It is safe, reporting a prevalence of 0.4% allergy cases,41 does not produce tooth or tongue discolouration or taste disturbances42 and, unlike alcohol-based products, can be used when using electrocautery.43 Its effectiveness has been well demonstrated through many in vitro studies against multiple viruses, including SARS-CoV, MERS-CoV, and influenza virus A (H1N1).36, 40, 44

Recent investigations have proposed that 0.23% PVP-I mouthwash for at least 15 seconds before procedures may reduce salivary viral load,44 indicating its use in COVID-19-positive patients.4, 38, 45, 46

reference link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428696/


More information: Chuan Xu et al, Differential Effects of Antiseptic Mouth Rinses on SARS-CoV-2 Infectivity In Vitro, Pathogens (2021). DOI: 10.3390/pathogens10030272

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