High rates of shift workers gain weight and develop diabetes, which has been attributed to a mismatch between their internal clocks and their schedules, so researchers from the Perelman School of Medicine at the University of Pennsylvania created a related mismatch by altering the function of a molecule within the brains of mice that shortened their circadian rhythms from 24 to 21 hours.
“When the external world doesn’t match the internal body’s cycles, metabolism pays the price,” said the study’s senior author, Mitchell A. Lazar, MD, Ph.D., the director of Penn Medicine’s Institute for Diabetes, Obesity, and Metabolism, and the Ware Professor of Diabetes and Metabolic Diseases. “We saw this in our study, and we believe that this happens similarly when people work odd hours that don’t align with how human bodies are wired.”
Published today in Science Advances, the researchers led by Lazar and primary investigator Marine Adlanmerini, Ph.D., a post-doctoral researcher in Lazar’s lab, sought to explore circadian desynchrony, a theory in which a disruption or alteration to a person’s innate, internal clock leads to poor outcomes.
Shift workers – those who may work long hours, overnight, or with irregular rest periods in between work – are subject to this, which could be why they appear to be at higher risk for obesity, diabetes, and metabolic diseases including having a liver that retains more fat.
So to explore whether circadian desynchrony is a viable explanation for this, the researchers removed certain molecules called REV-ERB, which reside in the brain cells of mice, and seem to control the body’s internal clock, holding it around 24-hour cycles. When REV-ERB was deleted, it caused the mouse body clocks to run roughly three hours shorter, which the researchers determined by tracking their regular sleep/awake pattern.
While their body clocks ran faster, some of these mice were kept in a typical day’s 24-hour cycle, with 12 hours of light and 12 of dark. Those mice, when on their regular diet, were able to keep their weight in check.
Moreover, the mice who still had REV-ERB but were given the high-fat and sugar diet did not have the same high amounts of poor outcomes.
“One potential explanation is that the internal clock of the mice missing REV-ERB was running at odds with the 24-hour day, which led to metabolic stress on the body,” Lazar said.
A way that was “fixed” was when the researchers adjusted the length of the mice’s “day” in the lab to match their malfunctioning internal clock: 21-hour days with 10.5-hour cycles of light and dark to match their 21-hour internal clock. When this happened, the mice with the altered clocks no longer were as susceptible to the ill-effects of the unhealthy diet.
“This may be a lesson for how to prevent or reduce obesity and diabetes in shift workers,” Lazar explained. “For example, timing of meals to better match the shift worker’s own clock could be of benefit. That would also be consistent with a number of studies in mice and people that have suggested that eating at specific times of day may improve weight control and metabolism.”
Moving forward, Lazar, Adlanmerini, and their team feel that potentially finding biomarkers which could be tested for and indicate how a person’s internal clock is running would be key.
“Information like that could then be matched to decisions about when to eat, much as blood sugar monitoring can help a diabetic understand when they should be taking more insulin,” said Lazar.
Type 2 diabetes (T2D) is a growing global health problem, with skeletal muscle insulin resistance being a primary defect in the pathology of this disease. While the etiology of this disease is complex, perturbed sleep/wake rhythms from shift-work, sleep disorders, and social jet lag are associated with obesity, T2D, and related comorbidities (1–4), highlighting the critical role of the circadian timing system for metabolic health.
Cell autonomous circadian rhythms are generated by a transcription-translation autoregulatory feedback loop composed of transcriptional activators CLOCK and BMAL1 (ARNTL) and their target genes Period (PER), Cryptochrome (CRY), and REV-ERBα (NR1D1), which rhythmically accumulate and form a repressor complex that interacts with CLOCK and BMAL1 to inhibit transcription (5).
Disruption of the molecular clock in skeletal muscle leads to obesity and insulin resistance in mouse models (6–8). While disrupted circadian rhythms alter metabolism, the extent to which these processes are impaired in people with T2D is unknown.
Several lines of evidence suggest that the link between dysregulated molecular-clock activity and T2D or insulin resistance may be tissue dependent.
In white adipose tissue, the evidence is equivocal. For example, subcutaneous white adipose tissue biopsies showed no difference of rhythm and amplitude of core-clock (PER1, PER2, PER3, CRY2, BMAL1, and DBP), clock-related (REVERBα), and metabolic (PGC1α) genes between individuals with normal weight, obesity, or T2D over a time-course experiment (9).
Conversely, when the sleep/wake cycle and dietary regime are controlled, amplitude oscillations of core-clock genes and number of rhythmic genes are reduced in adipose tissue from people with T2D as compared with healthy, lean individuals (10). In human leukocytes collected over a time-course experiment, mRNA expression of BMAL1, PER1, PER2, and PER3 was lower in people with T2D as compared to nondiabetic individuals (11).
In addition, BMAL1, PER1, and PER3 mRNA expression in leukocytes collected from people with T2D is inversely correlated with hemoglobin A1C (HbA1c) levels, suggesting an association of molecular-clock gene expression with T2D and insulin resistance.
Furthermore, in pancreatic islets from individuals with T2D or healthy controls, PER2, PER3, and CRY2 mRNA expression is positively correlated with islet insulin content and plasma HbA1c levels (12). Thus, there may be tissue specificity of molecular-clock regulation, which contributes to clinical outcomes related to insulin sensitivity and T2D etiology. The underlying mechanisms regulating metabolic rhythmicity and, particularly, whether rhythmicity is lost in T2D remain incompletely understood.
At the cellular level, primary human myotubes maintain a circadian rhythm, with the amplitude of the circadian gene REV-ERBα correlating with the metabolic disease state of the donor groups (13). This apparent link between the skeletal muscle molecular clock and insulin sensitivity may be partly mediated by molecular-clock regulation of metabolic targets. Chromatin immunoprecipitation (ChIP) sequencing has revealed distinct skeletal muscle–specific BMAL1 and REV-ERBα cistromes (14), with prominent molecular clock–targeted pathways, including mitochondrial function and glucose/lipid/protein metabolism (14, 15).
Moreover, these metabolic pathways may participate in retrograde signaling to control aspects of the molecular clock. Pharmacological inhibition of DRP1, a key regulator of mitochondrial fission and metabolism, alters the period length of BMAL1 transcriptional activity in human fibroblasts (16).
However, the signals and the clock-derived alterations that govern the rhythmicity of metabolism remain incompletely understood. Despite the growing evidence that several metabolic pathways are under circadian control, it is not clear whether circadian rhythmicity of the intrinsic molecular clock is altered in T2D. Here, we determined whether circadian control of gene expression and metabolism is altered at the cellular level in skeletal muscle from individuals with T2D.
reference link :https://www.science.org/doi/10.1126/sciadv.abi9654
More information: Marine Adlanmerini et al, REV-ERB nuclear receptors in the suprachiasmatic nucleus control circadian period and restrict diet-induced obesity, Science Advances (2021). DOI: 10.1126/sciadv.abh2007