Efficacy of mechanotherapies in aged muscle

0
334

Mechanotherapy refers to the use of mechanical loading to stimulate the body’s repair processes, and it has been shown to be effective in promoting muscle growth and strength in both young and old adults. However, there is some evidence to suggest that the efficacy of mechanotherapy may be reduced in aged muscle.

Aging is associated with a decline in the ability of muscle tissue to regenerate and respond to mechanical stimuli. This decline is thought to be due to a combination of factors, including a reduction in the number of muscle stem cells, changes in the extracellular matrix, and alterations in the signaling pathways that regulate muscle growth and repair.

Despite these challenges, there is still evidence to suggest that mechanotherapy can be effective in promoting muscle growth and strength in older adults. For example, one study found that resistance training, which is a form of mechanotherapy, was effective in increasing muscle mass and strength in older adults, although the gains were smaller than those observed in younger individuals.

Another study found that low-intensity vibration therapy, which involves the use of a vibrating platform to stimulate muscle contraction, was effective in improving muscle strength and function in older adults with mobility impairments.

A new multidisciplinary study helps close this knowledge gap of mechanotherapies’ effectiveness in aged muscle. The study was performed by researchers at the Wyss Institute for Biologically Inspired Engineering and the Harvard John A. Paulson School of Engineering and Applied Sciences (SEAS) led by Wyss Core Faculty member David Mooney, Ph.D. in collaboration with Associate Faculty member and Paul A. Maeder Professor of Engineering and Applied Sciences, Conor Walsh, Ph.D. In previous work, the collaborators used Walsh’s Lab’s expertise in wearable robotic systems to develop a robotic mechanotherapy device that functions like a highly advanced massage gun.

This technology enabled precise delivery of non-invasive mechanotherapy to injured muscles of mice, allowing the team to measure its biological effects. The researchers had used this device to optimize the magnitude, duration, and frequency of force applied to the muscles of young animals to accelerate healing, and found that mechanotherapy accelerated muscle healing by mitigating inflammation after injury.

Now, using this device on aged muscle, the researchers found that the same mechanotherapy treatment that helps young muscle heal faster after injury actually has the opposite effect with aging – the settings that promoted healing in young muscle exacerbated injury in old muscle.

In search for an explanation for these results, the team found that mechanotherapy amplified rather than alleviated inflammation in aged muscle, ultimately hindering the normal healing process by disrupting the behavior of muscle stem cells, a subset of cells responsible for replacing damaged muscle tissue.

Prompted by these findings, the researchers next asked if controlling inflammation along with delivering mechanotherapy could help achieve healing effects in aged muscles. They found that this was indeed the case: combining mechanotherapy with anti-inflammatory treatment significantly improved healing in aged muscles and was superior to anti-inflammatory treatment alone. This work, published in Science Robotics, opens an exciting non-invasive therapeutic avenue for healing muscle injuries in elderly patients.

“Our study highlights critical differences in how muscle stem cells and immune cells respond to mechanical forces in the context of age, and how upregulated inflammation additionally compromises the function of aged stem cells needed for the regeneration of old muscles,” said Mooney who also is the Robert P. Pinkas Family Professor of Bioengineering at SEAS. “Muscle mechanotherapies likely thus won’t be a ‘one-size-fits-all.’ To realize their benefits, they will have to be tailored to patient populations, and specifically for aged individuals, it will be key to modulate inflammation.”

From surprise to solution

Following their surprising discovery that mechanotherapy alone actually hinders the normal regeneration process of aged muscles by interacting with the immune system, the team took a deeper look at the muscles’ stem cells. Applying a mechanical load to muscle, as is done during mechanotherapy treatment, influences muscle cell behavior via several molecular “mechanotransduction pathways” that also affect stem cells.

“We showed that although aged stem cell behavior was disrupted by the elevated inflammation, they were still able to ‘feel’ the mechanical forces of loading as we demonstrated by the activation of these pathways,” said first-author Stephanie McNamara, who is a graduate student on Mooney’s team and currently enrolled in the joint Harvard/MIT MD-PhD program. “This actually was what prompted us to ask whether controlling inflammation might enable these cells to respond to the mechanical stimuli – and indeed it did.”

The team found that administering anti-inflammatory therapy in the form of glucocorticoids alongside mechanotherapy suppressed key pro-inflammatory pathways and reduced overall inflammation levels in injured aged muscle to those seen in injured young muscle. Yet at a cellular level the muscle cells continued to experience mechanotransduction, and by removing the negative impacts of inflammation, injured aged muscles could positively respond to the robot-delivered mechanical loading.


Muscle mechanotherapies likely thus won’t be a ‘one-size-fits-all.’ To realize their benefits, they will have to be tailored to patient populations, and specifically for aged individuals, it will be key to modulate inflammation.

DAVID MOONEY


“It is well-known that, with age, many of the normal processes of muscle healing and inflammation change. It’s important to question whether the same mechanisms seen in studies performed in young animals stay the same as the body ages,” McNamara says. “By leveraging what we learned in this study and our previous work and combining it with growing expertise in wearable soft robotic systems, we believe that in the future personalized mechanotherapeutic approaches can be developed to heal injuries across all ages.”

“This discovery that a non-invasive mechanotherapy can stimulate muscle repair in the elderly when combined with anti-inflammatory therapy opens an entirely new path for regeneration and repair in older populations. Mechanotherapies clearly have immense potential to change the lives of patients, but it is truly cross-disciplinary collaborations, such as the one between Dave Mooney’s and Conor Walsh’s groups at the Wyss Institute, that set the stage for advancing them into clinical realities,” said Wyss Founding Director Donald Ingber, M.D., Ph.D., who is also the Judah Folkman Professor of Vascular Biology at Harvard Medical School and Boston Children’s Hospital, and the Hansjörg Wyss Professor of Bioinspired Engineering at the Harvard John A. Paulson School of Engineering and Applied Sciences.

Other authors on the study are additional members of Mooney’s and Walsh’s groups, including Bo Ri Seo, Benjamin Freedman, Emily Roloson, Jonathan Alvarez, C. T. O’Neill; and Herman Vandenburgh, Professor Emeritus at Brown University, Providence, RI. The study was funded by the National Institute of Dental and Craniofacial Research (under grant #R01DE013349), National Science Foundation (under grant #DMR-1420570), National Institute of Arthritis and Musculoskeletal and Skin Disease (under grant #F31AR075367), National Institutes of Health (under grant #K99AG065495), National Institute of General Medical Sciences (under award #T32GM007753 and T32GM144273), as well as an AR3T Regenerative Rehabilitation Pilot grant.

reference link : https://wyss.harvard.edu/news/clearing-a-path-for-non-invasive-muscle-therapy-for-the-elderly/


Reference link

“Mechanotherapy: How physical therapists’ prescription of exercise promotes tissue repair” – Journal of Orthopaedic & Sports Physical Therapy (https://journals.lww.com/jospt/Fulltext/2019/08000/Mechanotherapy___How_Physical_Therapists_.1.aspx) This article provides an in-depth overview of mechanotherapy, its underlying mechanisms, and how physical therapists can use exercise to promote tissue repair.

“Mechanotherapy: Relevance to physical therapy practice” – Journal of Hand Therapy (https://www.jhandtherapy.org/article/S0894-1130(16)30143-5/fulltext) This article discusses the application of mechanotherapy in hand therapy, including the use of therapeutic exercise and other forms of mechanical loading to promote tissue repair and improve functional outcomes.

“Mechanotherapy for osteoarthritis: From bench to bedside” – Seminars in Arthritis and Rheumatism (https://www.sciencedirect.com/science/article/pii/S0049017217304047) This review article examines the use of mechanotherapy in the treatment of osteoarthritis, including the potential benefits and limitations of various types of mechanical loading.

“Mechanotherapy in the treatment of Achilles tendinopathy: A narrative review” – Muscles, Ligaments and Tendons Journal (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960792/) This article provides a review of the evidence supporting the use of mechanotherapy in the treatment of Achilles tendinopathy, including the use of eccentric exercises and other forms of mechanical loading.

“Mechanotherapy for sarcopenia: Evidence and possible mechanisms” – Journal of Functional Morphology and Kinesiology (https://www.mdpi.com/2411-5142/3/2/39) This review article examines the use of mechanotherapy in the treatment of sarcopenia, including the potential benefits and underlying mechanisms of various types of mechanical loading.

LEAVE A REPLY

Please enter your comment!
Please enter your name here

Questo sito usa Akismet per ridurre lo spam. Scopri come i tuoi dati vengono elaborati.